Journal article Open Access

Impact of Diuretics on Metabolic Activity of Urogenital Tract Microbiota in Women

Amar Balihodžić; Nadira Ibrišimović Mehmedinović; Suada Tinjić; Semira Galijašević; Kamelija Madacki-Todorović; Izet Eminović; Lejla Hasanbegović; Mirza Ibrišimović


DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="URL">https://zenodo.org/record/5502326</identifier>
  <creators>
    <creator>
      <creatorName>Amar Balihodžić</creatorName>
      <affiliation>Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina</affiliation>
    </creator>
    <creator>
      <creatorName>Nadira Ibrišimović Mehmedinović</creatorName>
      <affiliation>Department of Chemistry, Faculty of Natural Science and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina.</affiliation>
    </creator>
    <creator>
      <creatorName>Suada Tinjić</creatorName>
      <affiliation>Gynecology Polyclinic "Korak do života", Tuzla, Bosnia and Herzegovina.</affiliation>
    </creator>
    <creator>
      <creatorName>Semira Galijašević</creatorName>
      <affiliation>Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.</affiliation>
    </creator>
    <creator>
      <creatorName>Kamelija Madacki-Todorović</creatorName>
      <affiliation>Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.</affiliation>
    </creator>
    <creator>
      <creatorName>Izet Eminović</creatorName>
      <affiliation>Faculty of Natural Science and Mathematics, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.</affiliation>
    </creator>
    <creator>
      <creatorName>Lejla Hasanbegović</creatorName>
      <affiliation>Biochemical-Immunological-Hemathological "Medical Laboratory" Ilidža, Sarajevo, Bosnia and Herzegovina.</affiliation>
    </creator>
    <creator>
      <creatorName>Mirza Ibrišimović</creatorName>
      <affiliation>Gynecology Polyclinic "Korak do života",Tuzla, Bosnia and Herzegovina and Sarajevo Medical School, University Sarajevo School of Science and Technology.</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Impact of Diuretics on Metabolic Activity of Urogenital Tract Microbiota in Women</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2021</publicationYear>
  <subjects>
    <subject>Additionally, the results suggest that enzyme aspart</subject>
    <subject subjectScheme="issn">2249-8958</subject>
    <subject subjectScheme="handle">100.1/ijeat.F29880810621</subject>
  </subjects>
  <contributors>
    <contributor contributorType="Sponsor">
      <contributorName>Blue Eyes Intelligence Engineering and Sciences Publication(BEIESP)</contributorName>
      <affiliation>Publisher</affiliation>
    </contributor>
  </contributors>
  <dates>
    <date dateType="Issued">2021-10-30</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="JournalArticle"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/5502326</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="ISSN" relationType="IsCitedBy" resourceTypeGeneral="JournalArticle">2249-8958</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.35940/ijeat.F2988.10110121</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Limited knowledge exists about the effects of commonly used diuretic medications on the human normal flora. Thus, we investigated potential stimulatory effects of diuretic drug furosemide on urogenital tract microbiota in women. Three strains of E. coli and C. albicans with different biofilm forming capacities were obtained from female patients diagnosed with urinary tract infections. All tested strains were treated with two different concentrations of furosemide drug, in comparison to non-treated strains as the negative control. At specific time intervals, samples were obtained from growing culture and analyzed for their proliferation rate, aspartyl proteinase excretion and biofilm formation ability. E. coli and C. albicans strains significantly increased their aspartyl proteinase excretion under furosemide treatment. This effect was frequently observed after 16 hours of incubation at 37oC. This drug has also increased the biofilm forming capacities of E. coli and C. albicans strains. Interestingly, both E. coli and C. albicans non-biofilm former strains, gained the capacity of biofilm formation when treated with furosemide at certain concentrations. E. coli control became a weak biofilm former after 48 hours of incubation, while non-biofilm former C. albicans strain became a weak biofilm former in dose-dependent fashion, after 48 hours incubation with furosemide in concentration of 0.1 mg/mL, and after 16 hours of incubation with furosemide in concentration of 0.5 mg/mL. Loop diuretic drug furosemide is able to increase the microbial virulence and turn commensal microbes into opportunistic pathogens. Additionally, the results suggest that enzyme aspart&lt;/p&gt;</description>
  </descriptions>
</resource>
56
31
views
downloads
Views 56
Downloads 31
Data volume 14.1 MB
Unique views 56
Unique downloads 29

Share

Cite as