10.1016/j.jneumeth.2015.11.009
https://zenodo.org/records/50122
oai:zenodo.org:50122
Macchi, Francesca
Francesca
Macchi
KU Leuven, Laboratory for Neurobiology and Gene Therapy, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium
Deleersnijder, Angélique
Angélique
Deleersnijder
KU Leuven, Laboratory for Neurobiology and Gene Therapy, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium
Van den Haute, Chris
Chris
Van den Haute
KU Leuven, Laboratory for Neurobiology and Gene Therapy, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium and Leuven Viral Vector Core, KU Leuven, B-3000 Leuven, Flanders, Belgium
Munck, Sebastien
Sebastien
Munck
KU Leuven, Department of Human Genetics, Flanders Interuniversity Institute of Biotechnology, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium
Pottel, Hans
Hans
Pottel
KU Leuven campus Kulak Kortrijk, Public health and primary care, interdisciplinary research facility Life Sciences, Etienne Sabbelaan 53, B-8500 Kortrijk, Flanders, Belgium
Michiels, Annelies
Annelies
Michiels
KU Leuven, Laboratory for Neurobiology and Gene Therapy, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium and Leuven Viral Vector Core, KU Leuven, B-3000 Leuven, Flanders, Belgium
Debyser, Zeger
Zeger
Debyser
Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, B-3000 Leuven, Flanders, Belgium
Gerard, Melanie
Melanie
Gerard
KU Leuven, Laboratory for Neurobiology and Gene Therapy, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium and KU Leuven campus Kulak Kortrijk, Laboratory of Biochemistry, interdisciplinary research facility Life Sciences, Etienne Sabbelaan 53, B-8500 Kortrijk, Flanders, Belgium
Baekelandt, Veerle
Veerle
Baekelandt
KU Leuven, Laboratory for Neurobiology and Gene Therapy, Kapucijnenvoer 33, B-3000 Leuven, Flanders, Belgium
High-content analysis of α-synuclein aggregation and cell death in a cellular model of Parkinson's disease.
Zenodo
2015
Aggregation
Alpha-synuclein
Cell death
High-content analysis
Parkinson's disease
2015-11-24
https://zenodo.org/communities/inmind
https://zenodo.org/communities/eu
Creative Commons Attribution 4.0 International
BACKGROUND: Alpha-synuclein (α-SYN) aggregates represent a key feature of Parkinson's disease, but the exact relationship between α-SYN aggregation and neurodegeneration remains incompletely understood. Therefore, the availability of a cellular assay that allows medium-throughput analysis of α-SYN-linked pathology will be of great value for studying the aggregation process and for advancing α-SYN-based therapies.
NEW METHOD: Here we describe a high-content neuronal cell assay that simultaneously measures oxidative stress-induced α-SYN aggregation and apoptosis.
RESULTS: We optimized an automated and reproducible assay to quantify both α-SYN aggregation and cell death in human SH-SY5Y neuroblastoma cells.
COMPARISON WITH EXISTING METHODS: Quantification of α-SYN aggregates in cells has typically relied on manual imaging and counting or cell-free assays, which are time consuming and do not allow a concurrent analysis of cell viability. Our high-content analysis method for quantification of α-SYN aggregation allows simultaneous measurements of multiple cell parameters at a single-cell level in a fast, objective and automated manner.
CONCLUSIONS: The presented analysis approach offers a rapid, objective and multiparametric approach for the screening of compounds and genes that might alter α-SYN aggregation and/or toxicity.
European Commission
10.13039/501100000780
278850
Imaging of Neuroinflammation in Neurodegenerative Diseases