Published December 16, 2010 | Version v1
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Data from: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria)

  • 1. University of Helsinki

Description

We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandian populations and yielded an average of six to nine alleles per locus (range = 1–30) depending on the population. Average observed heterozygosities in the two populations were 0.16 (range = 0–0.91) and 0.36 (range = 0–1).

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