#file to create a time calibrated tree using the program PATHd8
#tree is from GSI_Pyrruf.nex with outgroup removed (O_Pyrruf) and rooted with Gyrinidae

Sequence length = 2229;

(((((((((Dy_Acmed:0.12956299999999987,Dy_Acsem:0.1252049999999998):0.07868800000000009,Dy_Grlib:0.22636900000000004):0.07104899999999992,(Dy_Agsp.:0.16495100000000007,Dy_Ilsp.:0.2757479999999999):0.058227999999999946):0.02713700000000019,Dy_Lamac:0.3496400000000002,Dy_Rhbin:0.22867700000000024):0.015448999999999824,(Dy_Cosp.:0.16076699999999988,Dy_Nerot:0.1425240000000001):0.10913499999999998):0.018143999999999938,(Dy_Cehub:0.26057700000000006,((Dy_Decon:0.2951799999999998,Dy_Hypus:0.24534699999999976):0.05718000000000023,((Dy_Hepul:0.2467109999999999,Dy_Nedim:0.21435300000000002,Dy_Neund:0.17705399999999982):0.07243000000000022,(Dy_Hysay:0.2786249999999999,Dy_Hysp.:0.23749900000000013):0.0374810000000001):0.023824999999999985):0.02211799999999986):0.03764499999999993):0.062608,(Ha_Haimm:0.22010299999999994,Ha_HaspA:0.19589299999999987):0.13644699999999998):0.04590000000000005,No_Hyiri:0.5620860000000001):0.06072349999999993,((((Gy_Dicil:0.11136499999999994,Gy_Didis:0.07826899999999992):0.03691599999999995,((Gy_Diema:0.08985599999999994,Gy_Dinig:0.05929899999999977):0.04150900000000002,Gy_Dihor:0.09193699999999994):0.09087900000000015):0.09817300000000007,Gy_Gysp.:0.382393):0.06606200000000007,(Gy_Gigib:0.16246699999999992,(Gy_Gylat:0.131008,(Gy_Gysp3:0.12259900000000012,Gy_Gymar:0.08712700000000018):0.044571999999999834):0.03365299999999993):0.08952900000000019):0.06072349999999993);

mrca: Dy_Grlib, Dy_Acmed, minage=5.333;
mrca: Dy_Agsp., Dy_Ilsp., minage=23.03;
mrca: Dy_Rhbin, Dy_Lamac, minage=23.03;
mrca: Dy_Hysay, Dy_Hysp., minage = 23.03;
mrca: Dy_Nerot, Dy_Cosp., minage=5.333;
mrca: Gy_Gymar, No_Hyiri, fixage=219.8;
mrca: No_Hyiri, Dy_Acmed, minage=201.3;
mrca: Ha_Haimm, Ha_HaspA, minage=100.5;
mrca: Dy_Hysp., Dy_Acmed, maxage=145;
mrca: Dy_Hysp., Dy_Acmed, minage=66;

#dates from International Comission on Stratigraphy chart v2013/01
#Dytiscidae fossils from World Catalogue of Insects: vol 3
	#fossil Acilius and Graphoderus described from the Miocene p.264 to 265
	#fossil Agabus from Oligocene
	#fossil Laccophilus from Oligocene
	#fossil hydroporous from Oligocene
	#fossil from Nebrioporous from the Miocene
#minimum age of Gyrinidae (Upper Triassic) from Ponomarenko 1977 as cited by Beutel et al 2013 (used to constrain divergence time of Gyrinidae from Dytiscidae)
#age of Dytiscoidae in Upper triassic from Ponomarenko 1993 as cited by Beutel et al 2013
#min age of Haliplus from Prokin and Ponmarenke 2013, lower Cretaecous
#max age of Dytiscidae from Tinothonian-Berriasian fossils described in Prokin and Ren 2010 
#min age of crown group of Dytiscidae from Prokin and Ren 2010 (no extant genera from Mesozoic)