Dataset Open Access

Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster

Jayawal, Vivek; Jimenez, Jamie; Magie, Robert; Nguyen, Kien; Clifton, Bryan; Yeh, Shudan; Ranz, Jose M.; Jayaswal, Vivek


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nmm##2200000uu#4500</leader>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Evolutionary genomics</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Sdic</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">sperm displacement</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">species-specific gene</subfield>
  </datafield>
  <controlfield tag="005">20220530182505.0</controlfield>
  <datafield tag="500" ind1=" " ind2=" ">
    <subfield code="a">&lt;div class="o-metadata__file-usage-entry"&gt;Sperm counts in the reproductive tract of doubly-mated females&lt;div class="o-metadata__file-description"&gt;Assays were performed to evaluate how sperm dynamics in the reproductive tract of doubly-mated females differs when the second-to-mate experimental male is either possessing (Sdic+ ) or lacking (Sdic- ) the entire D. melanogaster-specific Sdic multigene family. Virgin females were first mated to a reference Sdic+ male and then to one of the two possible experimental males (Sdic+ or 
 Sdic- ) two days later. Females were randomly assigned to one of three time points after the end of the second mating period (30 min, 1 h and 45 min, and 2 days) and subsequently flash frozen. Female reproductive tracts were dissected and the sperm from the different males were counted using a fluorescent microscope. Reference and experimental sperm fluoresce red and green, respectively.Sperm counting was performed for the bursa and both sperm storage organs, i.e. the paired spermatheca and the seminal receptacle.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;Sperm counts in doubly-mated females.xlsx&lt;br&gt;&lt;/div&gt;&lt;/div&gt;&lt;p&gt;Funding provided by: National Science Foundation&lt;br&gt;Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001&lt;br&gt;Award Number: MCB-1157876&lt;/p&gt;</subfield>
  </datafield>
  <controlfield tag="001">4952894</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of California, Irvine</subfield>
    <subfield code="a">Jimenez, Jamie</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of California, Irvine</subfield>
    <subfield code="a">Magie, Robert</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of California, Irvine</subfield>
    <subfield code="a">Nguyen, Kien</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of California, Irvine</subfield>
    <subfield code="a">Clifton, Bryan</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">National Central University</subfield>
    <subfield code="a">Yeh, Shudan</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of California, Irvine</subfield>
    <subfield code="a">Ranz, Jose M.</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of Sydney</subfield>
    <subfield code="a">Jayaswal, Vivek</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">15156</subfield>
    <subfield code="z">md5:007c913a0a39c6ff5ea82c4abba8f211</subfield>
    <subfield code="u">https://zenodo.org/record/4952894/files/Sperm counts in doubly-mated females.xlsx</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2017-12-13</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire_data</subfield>
    <subfield code="p">user-dryad</subfield>
    <subfield code="o">oai:zenodo.org:4952894</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">University of California, Irvine</subfield>
    <subfield code="a">Jayawal, Vivek</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-dryad</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/publicdomain/zero/1.0/legalcode</subfield>
    <subfield code="a">Creative Commons Zero v1.0 Universal</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">Sperm competition is a post-copulatory sexual selection mechanism in species in which females mate with multiple males.  Despite its evolutionary relevance in shaping male traits, the genetic mechanisms underlying sperm competition are poorly understood.  A recently originated multigene family specific to D. melanogaster, Sdic, is important for the outcome of sperm competition in doubly-mated females, although the mechanistic nature of this phenotype remained unresolved.  Here we compared doubly-mated females, second mated to either Sdic knockout or non-knockout males, and directly visualize sperm dynamics in the female reproductive tract.  We found that a less effective removal of first-to-mate male's sperm within the female's sperm storage organs is consistent with a reduced sperm competitive ability of the Sdic knockout males.  Our results highlight the role young genes can play in driving the evolution of sperm competition.</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isCitedBy</subfield>
    <subfield code="a">10.1111/evo.13417</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5061/dryad.j1t17</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">dataset</subfield>
  </datafield>
</record>
6
1
views
downloads
Views 6
Downloads 1
Data volume 15.2 kB
Unique views 6
Unique downloads 1

Share

Cite as