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Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster

Jayawal, Vivek; Jimenez, Jamie; Magie, Robert; Nguyen, Kien; Clifton, Bryan; Yeh, Shudan; Ranz, Jose M.; Jayaswal, Vivek


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    "description": "Sperm competition is a post-copulatory sexual selection mechanism in species in which females mate with multiple males.  Despite its evolutionary relevance in shaping male traits, the genetic mechanisms underlying sperm competition are poorly understood.  A recently originated multigene family specific to D. melanogaster, Sdic, is important for the outcome of sperm competition in doubly-mated females, although the mechanistic nature of this phenotype remained unresolved.  Here we compared doubly-mated females, second mated to either Sdic knockout or non-knockout males, and directly visualize sperm dynamics in the female reproductive tract.  We found that a less effective removal of first-to-mate male's sperm within the female's sperm storage organs is consistent with a reduced sperm competitive ability of the Sdic knockout males.  Our results highlight the role young genes can play in driving the evolution of sperm competition.", 
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    "title": "Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster", 
    "notes": "<div class=\"o-metadata__file-usage-entry\">Sperm counts in the reproductive tract of doubly-mated females<div class=\"o-metadata__file-description\">Assays were performed to evaluate how sperm dynamics in the reproductive tract of doubly-mated females differs when the second-to-mate experimental male is either possessing (Sdic+ ) or lacking (Sdic- ) the entire D. melanogaster-specific Sdic multigene family. Virgin females were first mated to a reference Sdic+ male and then to one of the two possible experimental males (Sdic+ or \n Sdic- ) two days later. Females were randomly assigned to one of three time points after the end of the second mating period (30 min, 1 h and 45 min, and 2 days) and subsequently flash frozen. Female reproductive tracts were dissected and the sperm from the different males were counted using a fluorescent microscope. Reference and experimental sperm fluoresce red and green, respectively.Sperm counting was performed for the bursa and both sperm storage organs, i.e. the paired spermatheca and the seminal receptacle.</div><div class=\"o-metadata__file-name\">Sperm counts in doubly-mated females.xlsx<br></div></div><p>Funding provided by: National Science Foundation<br>Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001<br>Award Number: MCB-1157876</p>", 
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    "keywords": [
      "Evolutionary genomics", 
      "Sdic", 
      "sperm displacement", 
      "species-specific gene"
    ], 
    "publication_date": "2017-12-13", 
    "creators": [
      {
        "affiliation": "University of California, Irvine", 
        "name": "Jayawal, Vivek"
      }, 
      {
        "affiliation": "University of California, Irvine", 
        "name": "Jimenez, Jamie"
      }, 
      {
        "affiliation": "University of California, Irvine", 
        "name": "Magie, Robert"
      }, 
      {
        "affiliation": "University of California, Irvine", 
        "name": "Nguyen, Kien"
      }, 
      {
        "affiliation": "University of California, Irvine", 
        "name": "Clifton, Bryan"
      }, 
      {
        "affiliation": "National Central University", 
        "name": "Yeh, Shudan"
      }, 
      {
        "affiliation": "University of California, Irvine", 
        "name": "Ranz, Jose M."
      }, 
      {
        "affiliation": "University of Sydney", 
        "name": "Jayaswal, Vivek"
      }
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