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Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster

Jayawal, Vivek; Jimenez, Jamie; Magie, Robert; Nguyen, Kien; Clifton, Bryan; Yeh, Shudan; Ranz, Jose M.; Jayaswal, Vivek


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  <identifier identifierType="URL">https://zenodo.org/record/4952894</identifier>
  <creators>
    <creator>
      <creatorName>Jayawal, Vivek</creatorName>
      <givenName>Vivek</givenName>
      <familyName>Jayawal</familyName>
      <affiliation>University of California, Irvine</affiliation>
    </creator>
    <creator>
      <creatorName>Jimenez, Jamie</creatorName>
      <givenName>Jamie</givenName>
      <familyName>Jimenez</familyName>
      <affiliation>University of California, Irvine</affiliation>
    </creator>
    <creator>
      <creatorName>Magie, Robert</creatorName>
      <givenName>Robert</givenName>
      <familyName>Magie</familyName>
      <affiliation>University of California, Irvine</affiliation>
    </creator>
    <creator>
      <creatorName>Nguyen, Kien</creatorName>
      <givenName>Kien</givenName>
      <familyName>Nguyen</familyName>
      <affiliation>University of California, Irvine</affiliation>
    </creator>
    <creator>
      <creatorName>Clifton, Bryan</creatorName>
      <givenName>Bryan</givenName>
      <familyName>Clifton</familyName>
      <affiliation>University of California, Irvine</affiliation>
    </creator>
    <creator>
      <creatorName>Yeh, Shudan</creatorName>
      <givenName>Shudan</givenName>
      <familyName>Yeh</familyName>
      <affiliation>National Central University</affiliation>
    </creator>
    <creator>
      <creatorName>Ranz, Jose M.</creatorName>
      <givenName>Jose M.</givenName>
      <familyName>Ranz</familyName>
      <affiliation>University of California, Irvine</affiliation>
    </creator>
    <creator>
      <creatorName>Jayaswal, Vivek</creatorName>
      <givenName>Vivek</givenName>
      <familyName>Jayaswal</familyName>
      <affiliation>University of Sydney</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2017</publicationYear>
  <subjects>
    <subject>Evolutionary genomics</subject>
    <subject>Sdic</subject>
    <subject>sperm displacement</subject>
    <subject>species-specific gene</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2017-12-13</date>
  </dates>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4952894</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsCitedBy">10.1111/evo.13417</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.5061/dryad.j1t17</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/dryad</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/publicdomain/zero/1.0/legalcode">Creative Commons Zero v1.0 Universal</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Sperm competition is a post-copulatory sexual selection mechanism in species in which females mate with multiple males.  Despite its evolutionary relevance in shaping male traits, the genetic mechanisms underlying sperm competition are poorly understood.  A recently originated multigene family specific to D. melanogaster, Sdic, is important for the outcome of sperm competition in doubly-mated females, although the mechanistic nature of this phenotype remained unresolved.  Here we compared doubly-mated females, second mated to either Sdic knockout or non-knockout males, and directly visualize sperm dynamics in the female reproductive tract.  We found that a less effective removal of first-to-mate male's sperm within the female's sperm storage organs is consistent with a reduced sperm competitive ability of the Sdic knockout males.  Our results highlight the role young genes can play in driving the evolution of sperm competition.</description>
    <description descriptionType="Other">&lt;div class="o-metadata__file-usage-entry"&gt;Sperm counts in the reproductive tract of doubly-mated females&lt;div class="o-metadata__file-description"&gt;Assays were performed to evaluate how sperm dynamics in the reproductive tract of doubly-mated females differs when the second-to-mate experimental male is either possessing (Sdic+ ) or lacking (Sdic- ) the entire D. melanogaster-specific Sdic multigene family. Virgin females were first mated to a reference Sdic+ male and then to one of the two possible experimental males (Sdic+ or 
 Sdic- ) two days later. Females were randomly assigned to one of three time points after the end of the second mating period (30 min, 1 h and 45 min, and 2 days) and subsequently flash frozen. Female reproductive tracts were dissected and the sperm from the different males were counted using a fluorescent microscope. Reference and experimental sperm fluoresce red and green, respectively.Sperm counting was performed for the bursa and both sperm storage organs, i.e. the paired spermatheca and the seminal receptacle.&lt;/div&gt;&lt;div class="o-metadata__file-name"&gt;Sperm counts in doubly-mated females.xlsx&lt;br&gt;&lt;/div&gt;&lt;/div&gt;&lt;p&gt;Funding provided by: National Science Foundation&lt;br&gt;Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001&lt;br&gt;Award Number: MCB-1157876&lt;/p&gt;</description>
  </descriptions>
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