BNT162b2 Vaccine: possible codons misreading, errors in protein synthesis and alternative splicing’s anomalies

The BioNTech/Pfizer BNT162b2 vaccine against Covid-19 is composed of an RNA having 4284 nucleotides , divided into 6 sections , which bring the information to create a factory of S Spike proteins , the ones used by Sars-CoV-2 (Covid-19) to infect the subject. After that, these proteins are directed outside the cell , triggering the immune reaction and antibody production. The problem is the heavy alteration of the mRNA : the Uracil is replaced to fool the immune system, the letters of all codon triplets are replaced by a C or a G , to extremely increase the speed of protein production , replacement of some amino acids with Proline , the addition of a sequence (3'-UTR), combined with anomalies of alternative splicing , which is the possibility of errors in translation of the sequence and synthesis of proteins ; they are not produced equal, but slightly different. All this can be the cause of many hereditary diseases and various types of tumors , from appearance to their growth, up to the metastasis formation. protein S Spike : just a transcription error , misreading codons (codon bias) wrong production of amino acids, then proteins, to cause serious long-term damage to health, the DNA is not modified , in the nucleus in the the modified mRNA sequence remain obscure.


Hints on how vaccine works
BioNTec/Pfizer's Sars-CoV-2 (Covid-19) vaccine called BNT162b2, but also called Tozinameran, or Comirnaty, contains about 30 mcg of RNA 1 , which is injected into a lipid sphere inside the human body, specifically inside the cytoplasm of cells, but outside the nucleus (where the DNA is located); this RNA has modified genetic information (hence modRNA), i.e. an mRNA (messenger RNA) containing instructions to set in place a factory of proteins, clones of the protein S Spike, i.e. the protein (and only the protein, not the whole virus) used by Covid-19 to enter the host and infect it. Once they are serially produced by the ribosomes, they are transported outside the cell, beyond the lipid coating; in this way the immune system identifies these proteins as cell invaders and attacks them, through the production of antibodies. This is why it is not conceivable that the vaccine induces Covid-19, or that it modifies human DNA.

Genetic sequence analysis
The vaccine is composed of 4284 nucleotides, divided into 6 sections: cap is the beginning of the sequence, which opens with the two GA nucleotides, falsely indicating that the mRNA comes from the human cell and thus be accepted 1 ; 5' indicates the direction to be followed for translation, while UTR indicates the area where the ribosome must rest in order to manufacture proteins. In this section, the U of Uracil has been replaced with a molecule of 1-methyl-3'pseudouridine, indicated with the character Ψ, to bypass the immune system and prevent the degradation of the RNA that has just entered; however, this is a factor that can lead to errors in the production of proteins. Multiple Ψ synthases are involved in the modification of specific positions, and defects in several of them are linked to human disease 1 Then there is the sig section, called the extended startup sequence of the S-glycoprotein signaling peptide, whose information is needed to guide the newly formed protein out of the cell via the endoplasmic reticulum; here too are put in place changes to the triplets of nucleotides to make the RNA accepted by the immune system, changing the position of some letters that make up the information with others (usually in 3rd position, "wobble"), apparently "harmless synonyms" (mainly by increasing the number of letters C and G, which encode the speed of production of proteins), but asparagine. However, while they specify identical amino acids, the two synonyms are not precisely the same, at least when it comes to the act of translation. Mechanistic studies show that there are subtle but significant differences in how each interacts with its corresponding transfer RNA (tRNA), differences that affect both the speed and the accuracy of translation. 2 While it is true that 3 letters form a codon and more than one codon encodes for the same amino acid, it is also true that by disproportionately increasing the rate of protein production and making at least one change to all triplets, one risks serious translation errors.
Also the characters that compose the sequence related to the construction of the real Spike protein S protein_mut have been altered with more C and G that was possible to add, respecting the synonyms in the standard genetic code table, with substitution of the amino acids Lysine (AAA) and Valine (GUU) with Proline (CUU), to prevent the constructed protein to collapse on itself. At the end of this sequence there are 2 stop codons. It is not fully proven that the same elements will be formed with this substitution and won't be misreading errors.

3'-UTR (Untranslated Region 3 First):
it should indicate the direction of translation of the sequence and improve protein synthesis, however many of its functions remain unknown; therefore it is impossible to ascertain its safety. What is known is stated by WHO and is the following sentence: the 3' UTR for the BioNTech/Pfizer vaccine was taken from "the aminoterminal enhancer of split (AES) mRNA and the mitochondrial encoded 12S ribosomal RNA". poly(A): we then reach the end of the sequence and encounter 30 A's, then a 10-nucleotide GCAUAUGACU linkage, followed by another 70 A's, since each mRNA can be reused by the organism multiple times.
When the A's run out, the mRNA is degraded.
All of these are proprietary modifications to increase protein expression, of which nothing is known about the actual translation implemented by the organism.
There is a strictly correlation with codons misreading (codon bias).

Investigation method Hints on proteins synthesis
Translation is generally divided into three phases: beginning, lengthening, and ending. 2

1.
The ribosome binds to the mRNA at the start codon; 2.
The polypeptide chain elongates in one direction of ribosome movement, by successive addition of amino acids;

3.
When a Stop codon is found, the polypeptide is released and the ribosome dissociates.

Alternative splicing and other errors
Another related problem is that the same pre-mRNA can give rise to different mature mRNAs, and therefore to slightly different proteins (alternative splicing). An alteration in the process of protein synthesis has been found to be the cause of the development and growth of some cancers, and other diseases, without altering the DNA in any way. Specifically, the genesis of 200 hereditary diseases and 33 types of cancer are implicated, in addition to neurodegenerative deseases. All splicing events identified in the three PHT series genes involve the loss of the messenger sequence reading frame, and the introduction of a Premature Termination Codon (PTC) always located more than 50-55 nucleotides upstream of the last exon-exon junction, which makes the alternative transcripts targets of the NMD (Nonsense-mediated mRNA Decay) surveillance system. For human and rat slc15a4/PHT1, this was demonstrated by NMD inhibition experiments in different cell lines, in which the expression of alternative variants to canonical transcripts was always stabilized following inhibition. 3

Conclusions Possible long-term risks on human health
The correlation between the speed of protein synthesis, increased by 100%, with the translation errors of the sequence, as well as the mechanism that affects the production of amino acids remain in this case for now obscure, being many trials owned by BioNTech/Pfizer.
Basically it can be said that the code of the total sequence is intrinsically altered in an unbalanced way, too much compared to the natural counterpart, and too much to be able to say that the human organism reproduces exactly the S Spike proteins, equal to each other, thus risking to bring serious damage to human health in the long term.
What will be produced from that sequence is far from well defined, but it is written in the genes of each individual, by ribosomial profile, how it will be translated and what will be produced, thus the benefits or damages that will be caused.