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PURPOSE:
\n\nThe aim of this paper is to present a simple and quantitative data analysis method with a new potential in the application of liver single-photon emission computed tomography (SPECT) imaging. We have established quantitative SPECT/computed tomography (CT) in vivo imaging protocols for determination of liver tumor burden based on the known role of Kupffer cells in cancer of the liver.
\n\nPROCEDURES:
\n\nAs it is also known that functional Kupffer cells accumulate particulate material contained in the arterial blood of liver supply, we used radiolabeled macro-aggregated albumin particles ([(99m)Tc]-MAA) injected intravenously to image liver disease. Quantification of cold spot liver lesion imaging was also a general objective.
\n\nMETHODS:
\n\nWe examined a healthy control group (BALB/C mice, n = 6) and group of induced hepatocellular carcinoma (HCC, matrilin-2 transgenic KO mice, n = 9), where hepatocellular carcinoma was induced by diethylnitrosamine. We used [(99m)Tc]-MAA as radiopharmaceutical for liver SPECT imaging in a small animal SPECT/CT system. A liver radioactivity overview map was generated. Segmentation of the liver was calculated by Otsu thresholding method. Based on the segmentation the radioactivity volume and the summarized liver activity were determined.
\n\nRESULTS:
\n\nTumor burden of the livers was quantitatively determined by creating parametric data from the resulting volumetric maps. Ex vivo liver mass data were applied for the validation of in vivo measurements. An uptake with cold spots as tumors was observed in all diseased animals in SPECT/CT scans. Isotope-labeled particle uptake (standardized uptake concentration) of control (median 0.33) and HCC (median 0.18) groups was significantly different (p = 0.0015, Mann Whitney U test).
\n\nCONCLUSION:
\n\nA new potential application of [(99m)Tc]-MAA was developed and presents a simple and very effective means to quantitatively characterize liver cold spot lesions resulting from Kupffer cell dysfunctions as a consequence of tumor burden.
", "funding": [ { "award": { "acronym": "INMIND", "id": "00k4n6c32::278850", "identifiers": [ { "identifier": "https://cordis.europa.eu/projects/278850", "scheme": "url" } ], "number": "278850", "program": "FP7", "title": { "en": "Imaging of Neuroinflammation in Neurodegenerative Diseases" } }, "funder": { "id": "00k4n6c32", "name": "European Commission" } } ], "publication_date": "2013-08-31", "publisher": "Zenodo", "resource_type": { "id": "publication-article", "title": { "de": "Zeitschriftenartikel", "en": "Journal article" } }, "rights": [ { "description": { "en": "The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited." }, "icon": "cc-by-icon", "id": "cc-by-4.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/licenses/by/4.0/legalcode" }, "title": { "en": "Creative Commons Attribution 4.0 International" } } ], "subjects": [ { "subject": "hepatocellular carcinoma (HCC)" }, { "subject": "SPECT/CT" }, { "subject": " quantification" }, { "subject": "particle" }, { "subject": "macro-aggregated albumin" }, { "subject": "Kupffer cell" } ], "title": "Quantitative liver lesion volume determination by nanoparticle-based SPECT." }, "parent": { "access": { "owned_by": { "user": 1964 } }, "communities": { "default": "724cc757-0e37-4b01-a81f-b8aa5f1d7b44", "entries": [ { "access": { "member_policy": "open", "record_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": false }, "created": "2014-01-06T13:42:25+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "724cc757-0e37-4b01-a81f-b8aa5f1d7b44", "links": {}, "metadata": { "curation_policy": "all uploads acknowledging the INMiND project are accepted", "page": "The goal of the EC FP7 Collaborative Project INMiND (GA 278850) is to carry out collaborative research on molecular mechanisms that link neuroinflammation with neurodegeneration in order to identify novel biological targets for activated microglia, which may serve for both diagnostic and therapeutic purposes, and to translate this knowledge into clinical application and patient benefit.
\r\n\r\n\r\n\r\n
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\r\n\r\n(ii) to identify and implement new targets for activated microglia, which may serve for diagnostic (imaging) and therapeutic purposes;
\r\n\r\n(iii) to design new molecular probes (tracers) for these novel targets and to implement and validate them in in vivo innovative model systems and in patients;
\r\n\r\n(iv) to image and quantify modulated microglia activity in patients undergoing immune therapy for cognitive impairment, and to relate the findings to clinical outcome.
\r\n\r\nThe INMiND projects brings together a group of excellent basic scientists and clinicians with a proven background in efficiently accomplishing common scientific goals (FP6 DiMI, www.dimi.eu), who belong to highly complementary fields of research (from genome-oriented to imaging scientists and clinicians), and who are dedicated to formulate novel image-guided therapeutic strategies for neuroinflammation-related neurodegenerative diseases.
\r\n\r\nThe strength of the project is that, across Europe, it coordinates research and training activities related to neuroinflammation, neurodegeneration, neuroregeneration, and imaging with special emphasis on translating basic mechanisms into clinical applications that shall provide health benefits for our aging population.
\r\n\r\nWith its intellectual excellence and its crucial mass the INMiND consortium is playing a major role in the European Research Area and will gain European leadership in the creation of new image-guided diagnosis and therapy paradigms in patients with neurodegenerative diseases.
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