10.1007/s11307-013-0679-y
https://zenodo.org/records/48584
oai:zenodo.org:48584
Veres, Dániel S
Dániel S
Veres
Department of Biophysics and Radiation Biology, Semmelweis University, H-1094, Budapest, Hungary
Máthé, Domokos
Domokos
Máthé
CROmed Translational Research Centers, H-1047 Budapest, Hungary
Futó, Ildikó
Ildikó
Futó
Department of Biophysics and Radiation Biology, Semmelweis University, H-1094, Budapest, Hungary
Horváth, Ildikó
Ildikó
Horváth
Department of Biophysics and Radiation Biology, Semmelweis University, H-1094, Budapest, Hungary
Balázs, Ákos
Ákos
Balázs
Ist Department of Surgery, Semmelweis University, H-1094, Budapest, Hungary
Karlinger, Kinga
Kinga
Karlinger
Department of Radiology and Oncotherapy, Semmelweis University, H-1094, Budapest, Hungary
Szigeti, Krisztián
Krisztián
Szigeti
Department of Biophysics and Radiation Biology, Semmelweis University, H-1094, Budapest, Hungary
Quantitative liver lesion volume determination by nanoparticle-based SPECT.
Zenodo
2013
hepatocellular carcinoma (HCC)
SPECT/CT
quantification
particle
macro-aggregated albumin
Kupffer cell
2013-08-31
https://zenodo.org/communities/inmind
https://zenodo.org/communities/eu
Creative Commons Attribution 4.0 International
PURPOSE:
The aim of this paper is to present a simple and quantitative data analysis method with a new potential in the application of liver single-photon emission computed tomography (SPECT) imaging. We have established quantitative SPECT/computed tomography (CT) in vivo imaging protocols for determination of liver tumor burden based on the known role of Kupffer cells in cancer of the liver.
PROCEDURES:
As it is also known that functional Kupffer cells accumulate particulate material contained in the arterial blood of liver supply, we used radiolabeled macro-aggregated albumin particles ([(99m)Tc]-MAA) injected intravenously to image liver disease. Quantification of cold spot liver lesion imaging was also a general objective.
METHODS:
We examined a healthy control group (BALB/C mice, n = 6) and group of induced hepatocellular carcinoma (HCC, matrilin-2 transgenic KO mice, n = 9), where hepatocellular carcinoma was induced by diethylnitrosamine. We used [(99m)Tc]-MAA as radiopharmaceutical for liver SPECT imaging in a small animal SPECT/CT system. A liver radioactivity overview map was generated. Segmentation of the liver was calculated by Otsu thresholding method. Based on the segmentation the radioactivity volume and the summarized liver activity were determined.
RESULTS:
Tumor burden of the livers was quantitatively determined by creating parametric data from the resulting volumetric maps. Ex vivo liver mass data were applied for the validation of in vivo measurements. An uptake with cold spots as tumors was observed in all diseased animals in SPECT/CT scans. Isotope-labeled particle uptake (standardized uptake concentration) of control (median 0.33) and HCC (median 0.18) groups was significantly different (p = 0.0015, Mann Whitney U test).
CONCLUSION:
A new potential application of [(99m)Tc]-MAA was developed and presents a simple and very effective means to quantitatively characterize liver cold spot lesions resulting from Kupffer cell dysfunctions as a consequence of tumor burden.
European Commission
10.13039/501100000780
278850
Imaging of Neuroinflammation in Neurodegenerative Diseases