Guarino, Agostino
Fiore, Davide
di Bernardo, Mario
2019-08-15
<p> </p>
<p><strong>Abstract</strong></p>
<p>The genetic toggle switch is a MIMO control system that can be controlled by varying the concentrations of two inducer molecules, aTc and IPTG, to achieve a desired level of expression of the two genes it comprises. It has been shown in Lugagne et al., Nature Communication (2017) that this can be accomplished through an open-loop external control strategy where the two inputs are selected as mutually exclusive periodic pulse waves of appropriate amplitude and duty-cycle. In this paper, we use a recently derived average model of the genetic toggle switch subject to these inputs to synthesize new feedback control approaches that adjust the inputs duty-cycle in real-time via two different possible strategies, a model-based hybrid PI-PWM approach and a so-called Zero-Average dynamics (ZAD) controller. The controllers are validated in-silico via both deterministic and stochastic simulations (SSA) illustrating the advantages and limitations of each strategy.</p>
<p> </p>
<p> </p>
<p> </p>
<p> </p>
This is a preprint of the conference paper published in "2019 18th European Control Conference (ECC)"
https://doi.org/10.23919/ECC.2019.8795642
oai:zenodo.org:4836153
Zenodo
https://zenodo.org/communities/cosy-bio
https://zenodo.org/communities/eu
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
2019 18th European Control Conference (ECC), Naples, Italy, 25-28 June 2019
In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation
info:eu-repo/semantics/conferencePaper