Conference paper Open Access

In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation

Guarino, Agostino; Fiore, Davide; di Bernardo, Mario


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{
  "description": "<p>&nbsp;</p>\n\n<p><strong>Abstract</strong></p>\n\n<p>The genetic toggle switch is a MIMO control system that can be controlled by varying the concentrations of two inducer molecules, aTc and IPTG, to achieve a desired level of expression of the two genes it comprises. It has been shown in Lugagne et al., Nature Communication (2017) that this can be accomplished through an open-loop external control strategy where the two inputs are selected as mutually exclusive periodic pulse waves of appropriate amplitude and duty-cycle. In this paper, we use a recently derived average model of the genetic toggle switch subject to these inputs to synthesize new feedback control approaches that adjust the inputs duty-cycle in real-time via two different possible strategies, a model-based hybrid PI-PWM approach and a so-called Zero-Average dynamics (ZAD) controller. The controllers are validated in-silico via both deterministic and stochastic simulations (SSA) illustrating the advantages and limitations of each strategy.</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy", 
      "@type": "Person", 
      "name": "Guarino,\u00a0Agostino"
    }, 
    {
      "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy", 
      "@type": "Person", 
      "name": "Fiore,\u00a0Davide"
    }, 
    {
      "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol, U.K.", 
      "@type": "Person", 
      "name": "di Bernardo, Mario"
    }
  ], 
  "headline": "In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2019-08-15", 
  "url": "https://zenodo.org/record/4836153", 
  "@type": "ScholarlyArticle", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.23919/ECC.2019.8795642", 
  "@id": "https://doi.org/10.23919/ECC.2019.8795642", 
  "workFeatured": {
    "location": "Naples, Italy", 
    "@type": "Event", 
    "name": "2019 18th European Control Conference (ECC)"
  }, 
  "name": "In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation"
}
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