Conference paper Open Access

In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation

Guarino, Agostino; Fiore, Davide; di Bernardo, Mario


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    "description": "<p>&nbsp;</p>\n\n<p><strong>Abstract</strong></p>\n\n<p>The genetic toggle switch is a MIMO control system that can be controlled by varying the concentrations of two inducer molecules, aTc and IPTG, to achieve a desired level of expression of the two genes it comprises. It has been shown in Lugagne et al., Nature Communication (2017) that this can be accomplished through an open-loop external control strategy where the two inputs are selected as mutually exclusive periodic pulse waves of appropriate amplitude and duty-cycle. In this paper, we use a recently derived average model of the genetic toggle switch subject to these inputs to synthesize new feedback control approaches that adjust the inputs duty-cycle in real-time via two different possible strategies, a model-based hybrid PI-PWM approach and a so-called Zero-Average dynamics (ZAD) controller. The controllers are validated in-silico via both deterministic and stochastic simulations (SSA) illustrating the advantages and limitations of each strategy.</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>", 
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    "title": "In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation", 
    "notes": "This is a preprint of the conference paper published in \"2019 18th European Control Conference (ECC)\"", 
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    "publication_date": "2019-08-15", 
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        "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy", 
        "name": "Guarino,\u00a0Agostino"
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      {
        "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy", 
        "name": "Fiore,\u00a0Davide"
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      {
        "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol, U.K.", 
        "name": "di Bernardo, Mario"
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