Conference paper Open Access

In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation

Guarino, Agostino; Fiore, Davide; di Bernardo, Mario

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  "DOI": "10.23919/ECC.2019.8795642", 
  "title": "In-silico feedback control of a MIMO synthetic Toggle Switch via Pulse-Width Modulation", 
  "issued": {
    "date-parts": [
  "abstract": "<p>&nbsp;</p>\n\n<p><strong>Abstract</strong></p>\n\n<p>The genetic toggle switch is a MIMO control system that can be controlled by varying the concentrations of two inducer molecules, aTc and IPTG, to achieve a desired level of expression of the two genes it comprises. It has been shown in Lugagne et al., Nature Communication (2017) that this can be accomplished through an open-loop external control strategy where the two inputs are selected as mutually exclusive periodic pulse waves of appropriate amplitude and duty-cycle. In this paper, we use a recently derived average model of the genetic toggle switch subject to these inputs to synthesize new feedback control approaches that adjust the inputs duty-cycle in real-time via two different possible strategies, a model-based hybrid PI-PWM approach and a so-called Zero-Average dynamics (ZAD) controller. The controllers are validated in-silico via both deterministic and stochastic simulations (SSA) illustrating the advantages and limitations of each strategy.</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>\n\n<p>&nbsp;</p>", 
  "author": [
      "family": "Guarino,\u00a0Agostino"
      "family": "Fiore,\u00a0Davide"
      "family": "di Bernardo, Mario"
  "id": "4836153", 
  "note": "This is a preprint of the conference paper published in \"2019 18th European Control Conference (ECC)\"", 
  "event-place": "Naples, Italy", 
  "type": "paper-conference", 
  "event": "2019 18th European Control Conference (ECC)"
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