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Feedback control promotes synchronisation of the cell-cycle across a population of yeast cells

Perrino, Giansimone; Fiore, Davide; Napolitano, Sara; Galdi, Francesca; La Regina, Antonella; di Bernardo, Mario; di Bernardo, Diego


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    "description": "<p>&nbsp;</p>\n\n<p>Abstract</p>\n\n<p>The periodic process of cell replication by division, known as cell-cycle, is a natural phenomenon occurring asynchronously in any cell population. Here, we consider the problem of synchronising cell-cycles across a population of yeast cells grown in a microfluidics device. Cells were engineered to reset their cell-cycle in response to low methionine levels. Automated syringes enable changing methionine levels (control input) in the microfluidics device. However, the control input resets only those cells that are in a specific phase of the cell-cycle (G1 phase), while the others continue to cycle unperturbed. We devised a simplified dynamical model of the cell-cycle, inferred its parameters from experimental data and then designed two control strategies: (i) an open-loop controller based on the application of periodic stimuli; (ii) a closed-loop model predictive controller (MPC) that selects the sequence of control stimuli which maximises a synchronisation index. Both the proposed control strategies were validated in-silico, together with experimental validation of the open-loop strategy.</p>", 
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    "title": "Feedback control promotes synchronisation of the cell-cycle across a population of yeast cells", 
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    "publication_date": "2019-03-27", 
    "creators": [
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        "orcid": "0000-0001-8095-9139", 
        "affiliation": "Telethon Institute of Genetics and Medicine, Via Campi Flegrei 34, 80078 Pozzuoli, Italy", 
        "name": "Perrino, Giansimone"
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        "orcid": "0000-0002-4011-5836", 
        "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, Via Claudio 21, 80125 Naples, Italy", 
        "name": "Fiore, Davide"
      }, 
      {
        "orcid": "0000-0001-5040-3391", 
        "affiliation": "Telethon\u00a0Institute of Genetics and Medicine, Via Campi Flegrei 34, 80078\u00a0Pozzuoli, Italy. -Department of Chemical, Materials and Industrial\u00a0Production Engineering, University of Naples Federico II, Piazzale Tecchio\u00a080, 80125 Naples, Italy.", 
        "name": "Napolitano, Sara"
      }, 
      {
        "affiliation": "Telethon\u00a0Institute of Genetics and Medicine, Via Campi Flegrei 34, 80078\u00a0Pozzuoli, Italy. Department of Chemical, Materials and Industrial\u00a0Production Engineering, University of Naples Federico II, Piazzale Tecchio\u00a080, 80125 Naples, Italy.", 
        "name": "Galdi, Francesca"
      }, 
      {
        "affiliation": "Telethon\u00a0Institute of Genetics and Medicine, Via Campi Flegrei 34, 80078\u00a0Pozzuoli, Italy. - Department of Chemical, Materials and Industrial\u00a0Production Engineering, University of Naples Federico II, Piazzale Tecchio\u00a080, 80125 Naples, Italy.", 
        "name": "La Regina, Antonella"
      }, 
      {
        "orcid": "0000-0002-3329-0839", 
        "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, Via Claudio 21, 80125 Naples, Italy - Department of Engineering Mathematics, University of Bristol, University Walk, BS8 1TR Bristol, U.K.", 
        "name": "di Bernardo, Mario"
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        "affiliation": "Telethon\u00a0Institute of Genetics and Medicine, Via Campi Flegrei 34, 80078\u00a0Pozzuoli, Italy. - Department of Chemical, Materials and Industrial\u00a0Production Engineering, University of Naples Federico II, Piazzale Tecchio\u00a080, 80125 Naples, Italy.", 
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