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In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics

Shannon, Barbara; Zamora-Chimal, Criseida G.; Postiglione, Lorena; Salzano, Davide; Grierson, Claire S.; Marucci, Lucia; Savery, Nigel J.; di Bernardo, Mario


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{
  "description": "<p><strong>Abstract</strong></p>\n\n<p>We study both&nbsp;<em>in silico</em>&nbsp;and&nbsp;<em>in vivo</em>&nbsp;the real-time feedback control of a molecular titration motif that has been earmarked as a fundamental component of antithetic and multicellular feedback control schemes in&nbsp;<em>E.&nbsp;coli</em>. We show that an external feedback control strategy can successfully regulate the average fluorescence output of a bacterial cell population to a desired constant level in real-time. We also provide&nbsp;<em>in silico</em>&nbsp;evidence that the same strategy can be used to track a time-varying reference signal where the set-point is switched to a different value halfway through the experiment. We use the experimental data to refine and parametrize an&nbsp;<em>in silico</em>&nbsp;model of the motif that can be used as an error computation module in future embedded or multicellular control experiments.</p>\n\n<p>&nbsp;</p>\n\n<ul>\n</ul>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "DNA-Protein Interactions Unit, School of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
      "@type": "Person", 
      "name": "Shannon,\u00a0Barbara"
    }, 
    {
      "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
      "@type": "Person", 
      "name": "Zamora-Chimal,\u00a0Criseida G."
    }, 
    {
      "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K.", 
      "@type": "Person", 
      "name": "Postiglione,\u00a0Lorena"
    }, 
    {
      "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80125 Naples, Italy", 
      "@type": "Person", 
      "name": "Salzano,\u00a0Davide"
    }, 
    {
      "affiliation": "School of Biological Sciences, University of Bristol, Bristol BS8 1UH, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
      "@type": "Person", 
      "name": "Grierson,\u00a0Claire S."
    }, 
    {
      "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
      "@type": "Person", 
      "name": "Marucci,\u00a0Lucia"
    }, 
    {
      "affiliation": "DNA-Protein Interactions Unit, School of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
      "@type": "Person", 
      "name": "Savery,\u00a0Nigel J."
    }, 
    {
      "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K. -\u00a0 Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80125 Naples, Italy -\u00a0 BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
      "@type": "Person", 
      "name": "di Bernardo,\u00a0Mario"
    }
  ], 
  "headline": "In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2020-09-23", 
  "url": "https://zenodo.org/record/4822902", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1021/acssynbio.0c00105", 
  "@id": "https://doi.org/10.1021/acssynbio.0c00105", 
  "@type": "ScholarlyArticle", 
  "name": "In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics"
}
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