Preprint Open Access

In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics

Shannon, Barbara; Zamora-Chimal, Criseida G.; Postiglione, Lorena; Salzano, Davide; Grierson, Claire S.; Marucci, Lucia; Savery, Nigel J.; di Bernardo, Mario


JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/cc8c92c7-b5e3-496d-9f7e-75facf0e8279/Shannon%20et%20al%202020%20ACS%20.pdf"
      }, 
      "checksum": "md5:9aef46d7a30024b78a4204353b04687f", 
      "bucket": "cc8c92c7-b5e3-496d-9f7e-75facf0e8279", 
      "key": "Shannon et al 2020 ACS .pdf", 
      "type": "pdf", 
      "size": 708794
    }
  ], 
  "owners": [
    44090
  ], 
  "doi": "10.1021/acssynbio.0c00105", 
  "stats": {
    "version_unique_downloads": 11.0, 
    "unique_views": 7.0, 
    "views": 7.0, 
    "version_views": 8.0, 
    "unique_downloads": 11.0, 
    "version_unique_views": 8.0, 
    "volume": 7796734.0, 
    "version_downloads": 11.0, 
    "downloads": 11.0, 
    "version_volume": 7796734.0
  }, 
  "links": {
    "doi": "https://doi.org/10.1021/acssynbio.0c00105", 
    "latest_html": "https://zenodo.org/record/4822902", 
    "bucket": "https://zenodo.org/api/files/cc8c92c7-b5e3-496d-9f7e-75facf0e8279", 
    "badge": "https://zenodo.org/badge/doi/10.1021/acssynbio.0c00105.svg", 
    "html": "https://zenodo.org/record/4822902", 
    "latest": "https://zenodo.org/api/records/4822902"
  }, 
  "created": "2021-05-27T19:56:46.900111+00:00", 
  "updated": "2021-05-30T02:20:47.171943+00:00", 
  "conceptrecid": "4822901", 
  "revision": 4, 
  "id": 4822902, 
  "metadata": {
    "access_right_category": "success", 
    "doi": "10.1021/acssynbio.0c00105", 
    "description": "<p><strong>Abstract</strong></p>\n\n<p>We study both&nbsp;<em>in silico</em>&nbsp;and&nbsp;<em>in vivo</em>&nbsp;the real-time feedback control of a molecular titration motif that has been earmarked as a fundamental component of antithetic and multicellular feedback control schemes in&nbsp;<em>E.&nbsp;coli</em>. We show that an external feedback control strategy can successfully regulate the average fluorescence output of a bacterial cell population to a desired constant level in real-time. We also provide&nbsp;<em>in silico</em>&nbsp;evidence that the same strategy can be used to track a time-varying reference signal where the set-point is switched to a different value halfway through the experiment. We use the experimental data to refine and parametrize an&nbsp;<em>in silico</em>&nbsp;model of the motif that can be used as an error computation module in future embedded or multicellular control experiments.</p>\n\n<p>&nbsp;</p>\n\n<ul>\n</ul>", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "title": "In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics", 
    "journal": {
      "volume": "9", 
      "issue": "10", 
      "pages": "2617\u20132624", 
      "title": "ACS Omega"
    }, 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "4822901"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "4822902"
          }
        }
      ]
    }, 
    "grants": [
      {
        "code": "766840", 
        "links": {
          "self": "https://zenodo.org/api/grants/10.13039/501100000780::766840"
        }, 
        "title": "Control Engineering of Biological Systems for Reliable Synthetic Biology Applications", 
        "acronym": "COSY-BIO", 
        "program": "H2020", 
        "funder": {
          "doi": "10.13039/501100000780", 
          "acronyms": [], 
          "name": "European Commission", 
          "links": {
            "self": "https://zenodo.org/api/funders/10.13039/501100000780"
          }
        }
      }
    ], 
    "communities": [
      {
        "id": "cosy-bio"
      }
    ], 
    "publication_date": "2020-09-23", 
    "creators": [
      {
        "affiliation": "DNA-Protein Interactions Unit, School of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
        "name": "Shannon,\u00a0Barbara"
      }, 
      {
        "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
        "name": "Zamora-Chimal,\u00a0Criseida G."
      }, 
      {
        "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K.", 
        "name": "Postiglione,\u00a0Lorena"
      }, 
      {
        "affiliation": "Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80125 Naples, Italy", 
        "name": "Salzano,\u00a0Davide"
      }, 
      {
        "affiliation": "School of Biological Sciences, University of Bristol, Bristol BS8 1UH, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
        "name": "Grierson,\u00a0Claire S."
      }, 
      {
        "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
        "name": "Marucci,\u00a0Lucia"
      }, 
      {
        "affiliation": "DNA-Protein Interactions Unit, School of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K. - BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
        "name": "Savery,\u00a0Nigel J."
      }, 
      {
        "affiliation": "Department of Engineering Mathematics, University of Bristol, Bristol BS8 1UB, U.K. -\u00a0 Department of Electrical Engineering and Information Technology, University of Naples Federico II, 80125 Naples, Italy -\u00a0 BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol BS8 1TQ, U.K.", 
        "name": "di Bernardo,\u00a0Mario"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "subtype": "preprint", 
      "type": "publication", 
      "title": "Preprint"
    }
  }
}
7
11
views
downloads
Views 7
Downloads 11
Data volume 7.8 MB
Unique views 7
Unique downloads 11

Share

Cite as