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In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics

Shannon, Barbara; Zamora-Chimal, Criseida G.; Postiglione, Lorena; Salzano, Davide; Grierson, Claire S.; Marucci, Lucia; Savery, Nigel J.; di Bernardo, Mario


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    <dct:description>&lt;p&gt;&lt;strong&gt;Abstract&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;We study both&amp;nbsp;&lt;em&gt;in silico&lt;/em&gt;&amp;nbsp;and&amp;nbsp;&lt;em&gt;in vivo&lt;/em&gt;&amp;nbsp;the real-time feedback control of a molecular titration motif that has been earmarked as a fundamental component of antithetic and multicellular feedback control schemes in&amp;nbsp;&lt;em&gt;E.&amp;nbsp;coli&lt;/em&gt;. We show that an external feedback control strategy can successfully regulate the average fluorescence output of a bacterial cell population to a desired constant level in real-time. We also provide&amp;nbsp;&lt;em&gt;in silico&lt;/em&gt;&amp;nbsp;evidence that the same strategy can be used to track a time-varying reference signal where the set-point is switched to a different value halfway through the experiment. We use the experimental data to refine and parametrize an&amp;nbsp;&lt;em&gt;in silico&lt;/em&gt;&amp;nbsp;model of the motif that can be used as an error computation module in future embedded or multicellular control experiments.&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt; &lt;ul&gt; &lt;/ul&gt;</dct:description>
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