Preprint Open Access

# In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics

Shannon, Barbara; Zamora-Chimal, Criseida G.; Postiglione, Lorena; Salzano, Davide; Grierson, Claire S.; Marucci, Lucia; Savery, Nigel J.; di Bernardo, Mario

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{
"DOI": "10.1021/acssynbio.0c00105",
"container_title": "ACS Omega",
"title": "In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics",
"issued": {
"date-parts": [
[
2020,
9,
23
]
]
},
"abstract": "<p><strong>Abstract</strong></p>\n\n<p>We study both&nbsp;<em>in silico</em>&nbsp;and&nbsp;<em>in vivo</em>&nbsp;the real-time feedback control of a molecular titration motif that has been earmarked as a fundamental component of antithetic and multicellular feedback control schemes in&nbsp;<em>E.&nbsp;coli</em>. We show that an external feedback control strategy can successfully regulate the average fluorescence output of a bacterial cell population to a desired constant level in real-time. We also provide&nbsp;<em>in silico</em>&nbsp;evidence that the same strategy can be used to track a time-varying reference signal where the set-point is switched to a different value halfway through the experiment. We use the experimental data to refine and parametrize an&nbsp;<em>in silico</em>&nbsp;model of the motif that can be used as an error computation module in future embedded or multicellular control experiments.</p>\n\n<p>&nbsp;</p>\n\n<ul>\n</ul>",
"author": [
{
"family": "Shannon,\u00a0Barbara"
},
{
"family": "Zamora-Chimal,\u00a0Criseida G."
},
{
"family": "Postiglione,\u00a0Lorena"
},
{
"family": "Salzano,\u00a0Davide"
},
{
"family": "Grierson,\u00a0Claire S."
},
{
"family": "Marucci,\u00a0Lucia"
},
{
"family": "Savery,\u00a0Nigel J."
},
{
"family": "di Bernardo,\u00a0Mario"
}
],
"page": "2617\u20132624",
"volume": "9",
"type": "article",
"issue": "10",
"id": "4822902"
}
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