Preprint Open Access

A low-cost, open-source Turbidostat design for in-vivo control experiments in Synthetic Biology

Guarino, Agostino; Shannon, Barbara; Marucci, Lucia; Grierson, Claire; Savery, Nigel

Abstract

Continuous culture platforms are required to characterise the dynamics of new engineered systems in synthetic biology. In this paper, we review existing turbidostat platforms before describing the design and implementation of our new flexible and low-cost turbidostat for in-vivocontrol experiments. We provide preliminary experimental results on controlling the optical density of a bacterial culture. We also discuss the potential extensions to our design for the development of in-vivomulticellular control experiments.

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