SIMPLE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF METHOTREXATE AND CURCUMIN IN BULK DRUGS

A method of estimation for the simultaneous estimation of methotrexate and curcumin in their bulk powder is developed and validated as per ICH guidelines. The method involved measurement of absorbance of the methotrexate and curcumin at the 𝜆max of the drugs i.e., 307nm and 425nm in methanol. Calibration curves of methotrexate and curcumin were found to be linear in the range 2-18µg/mL and 1-7 µg/mL respectively. The correlation coefficient values R² was found to be 0.999. LOD and LOQ were 122.23ng/mL and 370.4ng/mL for methotrexate, 12.69ng/mL and 38.48ng/mL for curcumin. The percentage recovery at various concentration levels varied in between 99 to 101% for both the drugs estimated, thereby confirming accuracy of the method developed. In the precision study, the %RSD was found to be less than 2%, indicating the method is precise. Hence it can be concluded that the method developed is simple, accurate, precise, and economical.

Keywords:- Methotrexate, Curcumin, Simultaneous estimation and Validation.