Journal article Open Access

Glucan Particles Loaded with Fluorinated Emulsions: A sensitivity Improvement for the visualization of Phagocytic Cells by 19F-MRI.

Chirizzi, Cristina; Dastrù, Walter; Castelli, Daniela D; Menchise, Valeria; Aime, Sivio; Terreno, Enzo

A protocol to load perfluoro-crown-ether (PFCE) nanoemulsion directly into yeast-derived glucan particles (GPs) was developed. It was observed that the PFCE encapsulation did not affect the 19F-MRI properties of the nanoemulsion that is currently in clinical trials. GPs loaded with PFCE nanoemulsion were taken up avidly by murine macrophages in vitro, resulting in a cellular uptake 150
% higher than the not GPs-entrapped nanoemulsion. Accordingly, a corresponding improvement in the 19F-MRI detection of the labelled cells can be obtained. The high biotolerability and versatility of GPs, make these microcarriers a promising option for designing of improved in vivo cellular imaging protocols.

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