Abf1 TAP-tagged strain
Bas1-Myc
NoPi; W303 strain
HighPi; W303 strain
Genotype: Dal80-Myc; Conditions: grown in proline-containing medium;
myc-tagged Fhl1
Flag-tagged Fhl1
myc-tagged Fhl1 with deletion of forkhead domain (Fhl1-ΔFH-Myc)
Flag-tagged Fhl1 with deletion of forkhead domain (Fhl1-ΔFH-Flag)
Genotype: Gat1-Myc; Conditions: grown in proline-containing medium;
W303 (Z1256); GCN4-myc
W303 (Z1256); GCN4-myc
W303 (Z1256); GCN4-myc
W303 (Z1256); GCN4-myc; RTG3-knockout (co-factor)
W303 (Z1256); GCN4-myc; RTG3-knockout (co-factor)
W303 (Z1256); GCN4-myc; RTG3-knockout (co-factor)
strain: BY4741 MATa his3{delta}1 leu2{delta}0 met15{delta}0 ura3{delta}0 pEGH 2{mu} URA3 promGAL1-10-GST-GCN4
Treatment: sulfometuron added; Growth: for mid - long fase(OD600 about 0.6 to 0.8); Genotype: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Strain: BY4741
Treatment: without adding sulfometuron; Growth: for mid - long fase(OD600 about 0.6 -0.8); Genotype: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Strain: BY4741
control
heat shock
HSF1 TAP-tagged yeast cells; none; temperature: 30 C
HSF1 TAP-tagged yeast cells; none; temperature: 39 C
Treatment: non heat shock, 30 degrees; Genotype: Wildtype;
Treatment: 5 min heat shock, 39 degree; Genotype: Wildtype;
Treatment: 120 min heat shock, 39 degree; Genotype: Wildtype;
Ino4-Myc
time point 1; strain: BY5764: CEN.PK MATa
time point 2; strain: BY5764: CEN.PK MATa
time point 3; strain: BY5764: CEN.PK MATa
time point 4; strain: BY5764: CEN.PK MATa
time point 5; strain: BY5764: CEN.PK MATa
time point 6; strain: BY5764: CEN.PK MATa
Strain: 7072 and 7073 Genotype: MSN2::FLAG BRN1::myc, msn2 Type: All Replicates Merged data
time point 1; strain: BY5764: CEN.PK MATa
time point 2; strain: BY5764: CEN.PK MATa
time point 3; strain: BY5764: CEN.PK MATa
time point 4; strain: BY5764: CEN.PK MATa
time point 5; strain: BY5764: CEN.PK MATa
time point 6; strain: BY5764: CEN.PK MATa
yIN219 strain
yIN218 strain; Pichia pastoris Ndt80
Genotype: Flag-Nhp10 Source: Asynchronous growth in liquid YPD
Genotype: Flag-Nhp10 Source: Asynchronous growth in liquid YPD
NoPi; W303 strain
HighPi; W303 strain
NoPi; W303 strain
HighPi; W303 strain
Reb1 TAP-tagged strain
wild type (mock-treated by addition of YEPD)
wild type (YEPD + 0.4 M KCl for 5 minutes)
0 hrs after treatment with H2O2; Snt2-13Myc strain;
0.5 hrs after treatment with H2O2; Snt2-13Myc strain;
4 hrs after treatment with H2O2; Snt2-13Myc strain;
0 hrs after treatment with H2O2; untagged strain BY4741 (negative control for SNT2 and ECM5)
4 hrs after treatment with H2O2; untagged strain BY4741 (negative control for SNT2 and ECM5)
untreated (0 hour time-point); Snt2-13Myc strain
0.5 hrs after treatment with DMSO; Snt2-13Myc strain
0.5 hrs after treatment with Rapamycin; Snt2-13Myc strain
0.5 hrs after treatment with DMSO; Snt2-13Myc strain
4 hrs after treatment with Rapamycin; Snt2-13Myc strain
MATa, ura3-, leu2-3,112, trp1-1, his3-11,15, fpr1::NAT, RPL13A-2xFKBP12::TRP1, tor1-1, ade2-1, can1-100; YF2229 strain
MATalpha, ura3-, leu2-3,112, trp1-1, his3-11,15, fpr1::NAT, RPL13A-2xFKBP12::TRP1, tor1-1, ade2-1, can1-100, RPB1-FRB::HIS3MX; YSB3202 strain; Rpb1-AA_0min
MATalpha, ura3-, leu2-3,112, trp1-1, his3-11,15, fpr1::NAT, RPL13A-2xFKBP12::TRP1, tor1-1, ade2-1, can1-100, RPB1-FRB::HIS3MX; YSB3202 strain; Rpb1-AA_60min
MATalpha, ura3-, leu2-3,112, trp1-1, his3-11,15, fpr1::NAT, RPL13A-2xFKBP12::TRP1, tor1-1, ade2-1, can1-100, BDF1-FRB::HIS3MX; YSB3323 strain; Bdf1-AA_0min
MATalpha, ura3-, leu2-3,112, trp1-1, his3-11,15, fpr1::NAT, RPL13A-2xFKBP12::TRP1, tor1-1, ade2-1, can1-100, BDF1-FRB::HIS3MX; YSB3323 strain; Bdf1-AA_60min
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX SPT15-13xmyc-KanMX;
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 ABF1-FRB-KanMX SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 ABF1-FRB-KanMX SPT15-13xmyc-KanMX;
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 REB1-FRB-HIS3MX SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype:W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 REB1-FRB-HIS3MX SPT15-13xmyc-KanMX;
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 RAP1-FRB-RAP1-LEU2 SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 RAP1-FRB-RAP1-LEU2 SPT15-13xmyc-KanMX;
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX ABF1-FRB-KanMX SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX ABF1-FRB-KanMX SPT15-13xmyc-KanMX;
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX REB1-FRB-HIS3MX SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX REB1-FRB-HIS3MX SPT15-13xmyc-KanMX;
Treatment: no rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX RAP1-FRB-RAP1-LEU2 SPT15-13xmyc-KanMX;
Treatment: 60 min rapamycin; Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 STH1-FRB-KanMX RAP1-FRB-RAP1-LEU2 SPT15-13xmyc-KanMX;
Treatment: no rapamycin Strain: YSK426 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SNF2-FRB-KanMX; SPT15-13xmyc-KanMX
Treatment: 60 minutes rapamycin Strain: YSK426 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SNF2-FRB-KanMX; SPT15-13xmyc-KanMX
Treatment: no rapamycin Strain: YSK423 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 ISW2-FRB-HIS3MX; SPT15-13xmyc-KanMX
Treatment: 60 minutes rapamycin Strain: YSK423 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 ISW2-FRB-HIS3MX; SPT15-13xmyc-KanMX
Treatment: no auxin Strain: YSK492 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX; INO80-aid*-9myc-HphNT Padh1-Os.TIR1
Treatment: 30 minutes auxin (3-IAA) Strain: YSK492 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX; INO80-aid*-9myc-HphNT Padh1-Os.TIR1
Treatment: no auxin Strain: YSK491 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX; ISW1-aid*-9myc-HphNT Padh1-Os.TIR1
Treatment: 30 minutes auxin (3-IAA) Strain: YSK426 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX; ISW1-aid*-9myc-HphNT Padh1-Os.TIR1
Treatment: no rapamycin Strain: YSK474 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 CHD1-FRB-KanMX; SPT15-13xmyc-KanMX
Treatment: 60 minutes rapamycin Strain: YSK474 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 CHD1-FRB-KanMX; SPT15-13xmyc-KanMX
Treatment: no rapamycin/auxin Strain: YSK495 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX ISW2-FRB-HIS3MX; INO80-aid*-9myc-HphNT Padh1-Os.TIR1W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX ISW2-FRB-HIS3MX; INO80-aid*-9myc-HphNT Padh1-Os.TIR1
Treatment: 60 minutes rapamycin 30 minutes auxin Strain: YSK495 Genotype: W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX ISW2-FRB-HIS3MX; INO80-aid*-9myc-HphNT Padh1-Os.TIR1W303 tor1-1 fpr1::NAT RPL13A-2XFKB12::TRP1 SPT15-FLAG-HphMX ISW2-FRB-HIS3MX; INO80-aid*-9myc-HphNT Padh1-Os.TIR1
strain: ML1; Myc tagged Sum1
W303 (Z1256); SUT1-myc; NRG1-knockout (co-factor)
W303 (Z1256); SWI4-myc
W303 (Z1256); SWI4-myc; SWI6-knockout (co-factor)
W303-1A TBF1-13xMyc::HIS3
strain: S96 yrr1Δ (YRR1-knockout); allele: YRR1_YJM789; grown in YPD + 4NQO