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Data from: Concerted evolution reveals co-adapted amino acid substitutions in Na+K+ ATPase of frogs that prey on toxic toads

Mohammadi, Shabnam; Yang, Lu; Harpak, Arbel; Herrera-Álvarez, Santiago; Rodríguez-Ordoñez, María del Pilar; Peng, Julie; Zhang, Karen; Storz, Jay; Dobler, Susanne; Crawford, Andrew; Andolfatto, Peter

Gene duplication is an important source of evolutionary innovation, but the functional distinction between duplicates can be opposed by ongoing gene conversion between them. Here we document a tandem duplication of Na+,K+-ATPase subunit α1 (ATP1A1) sharedby frogs in the genus Leptodactylus,a group of species that feeds on toxic toads. One ATP1A1 paralog evolved resistance to toad toxins while the other paralog retained ancestral susceptibility. Frequent non-allelic gene conversion homogenized most of the paralog sequences,yet the two Leptodactylusparalogs are distinguished by 12 amino acid substitutions that were maintained by strong selection that counteracted gene conversion. Protein-engineering experiments show that two substitutions substantially increase toxin resistance, whereas the 10 additional substitutions mitigate deleterious pleiotropic effects on enzyme function. Our results highlight how neofunctionalized gene duplicates can help pinpoint functional substitutions and their interactions with the genetic backgrounds on which they arise.

"NKA Enzyme Assays Raw Data" - raw plate reader data and downstream calculations for enzyme assays used in this study

"Mohammadi_ATP1A1_alignment" - alignment of ATP1A1 gene sequences used to generate phylogenetic trees in this study

"NKA_Enzyme_Assays_Raw_Data_Readme" - ReadMe file for the enzyme assay raw data

Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: R01- GM115523

Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: R01- HL087216

Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number: OIA-1736249

Funding provided by: Deutsche Forschungsgemeinschaft
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659
Award Number: DO527/10-1

Funding provided by: Simons Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000893
Award Number: 633313

Files (94.4 kB)
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Mohammadi_ATP1A1_alignment.fasta
md5:f4acddde3efacd243033944ba6df019d 		41.0 kB Download
NKA_Enzyme_Assays_Raw_Data.xlsx
md5:87b376bd2be65027fa56d4582458114e 		49.2 kB Download
NKA_Enzyme_Assays_Raw_Data_Readme.txt
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Publication date:
April 22, 2021
DOI:
10.5281/zenodo.4677691

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