4664603
doi
10.5281/zenodo.4664603
oai:zenodo.org:4664603
Lisanne Lutter
Utrecht University Medical Center
Mara J.T. Nicolasen
Radboud University Medical Center
Martin ter Beest
Radboud University Medical Center
Sahar El Aidy
University of Groningen
Bas Oldenburg
Utrecht University Medical Center
Geert van den Bogaart
University of Groningen
Primary data manuscript Chromogranin A regulates gut permeability via the antagonistic actions of its proteolytic peptides
Elke Muntjewerff
Radboud University Medical Center
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
<p>Primary data of the publication 'Chromogranin A regulates gut permeability via the antagonistic actions of its proteolytic peptides', including: Microbiota analysis WT, CST-KO and CgA-KO mouse, Human colon electron microscopy, CASP3 stain WT and CST-KO mouse colon, F4/80 stain WT and CST-KO mouse colon. Full paper is published in Acta Physioloigica and can be accessed via: <a href="https://doi.org/10.1111/apha.13655">https://doi.org/10.1111/apha.13655</a></p>
<p><strong>Abstract paper</strong></p>
<p><strong>Aim </strong>A ‘leaky’ gut barrier has been implicated in the initiation and progression of a multitude of diseases, e.g., inflammatory bowel disease (IBD), irritable bowel syndrome, and celiac disease. Here we show how pro‐hormone Chromogranin A (CgA), produced by the enteroendocrine cells, and Catestatin (CST: hCgA<sub>352‐372</sub>), the most abundant CgA‐derived proteolytic peptide, affect the gut barrier.</p>
<p><strong>Methods </strong>Colon tissues from region‐specific CST‐knockout (CST‐KO) mice, CgA‐knockout (CgA‐KO) and WT mice were analyzed by immunohistochemistry, Western blot, ultrastructural and flowcytometry studies. FITC‐dextran assays were used to measure intestinal barrier function. Mice were supplemented with CST or CgA fragment pancreastatin (PST: CgA<sub>250‐301</sub>). The microbial composition of cecum was determined. CgA and CST levels were measured in blood of IBD patients.</p>
<p><strong>Results </strong>Plasma levels of CST were elevated in IBD patients. CST‐KO mice displayed (i) elongated tight, adherens junctions and desmosomes similar to IBD patients, (ii) elevated expression of Claudin 2, and (iii) gut inflammation. Plasma FITC‐dextran measurements showed increased intestinal paracellular permeability in the CST‐knockout mice. This correlated with a higher ratio of Firmicutes to Bacteroidetes, a dysbiotic pattern commonly encountered in various diseases. Supplementation of CST‐knockout mice with recombinant CST restored paracellular permeability and reversed inflammation, whereas CgA‐knockout mice supplementation with CST and/or PST in CgA‐KO mice showed that intestinal paracellular permeability is regulated by the antagonistic roles of these two peptides: CST reduces and PST increases permeability.</p>
<p><strong>Conclusion </strong>The pro‐hormone CgA regulates the intestinal paracellular permeability. CST is both necessary and sufficient to reduce permeability and primarily acts by antagonizing PST.</p>
G.v.d.B. is funded by a Young Investigator Grant from the Human Frontier Science Program (HFSP; RGY0080/2018) and a Vidi grant from the Netherlands Organisation for Scientific Research (NWO-ALW VIDI 864.14.001). G.v.d.B has also received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 862137).
Zenodo
2021-04-06
info:eu-repo/semantics/other
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