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Published March 26, 2021 | Version v1
Journal article Open

THE RP-UPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF LESINURAD AND ALLOPURINOL

Description

In order to develop a newer or improved analytical method, the analyst has to set some goals. The method should be precise to the drug under study. It is necessary to determine the analyte at trace levels accurately. The UPLC techniques have now become extremely reliable and indispensable. Lesinurad is an oral uric acid transporter 1 (URAT1) inhibitor indicated for the treatment of hyperuricemia associated with gout. Allopurinol is a xanthine oxidase enzyme inhibitor that is considered to be one of the most effective drugs used to decrease urate levels and is frequently used in the treatment of chronic gout. Run time was selected to be 2 min because the analysis gave peaks around 0.401 and 0.718 ±0.02 min of Lesinurad and Allopurionol. The percent recovery was found to be in between 98.0 to 102.0%. The analytical method was found to be linear over the range 50-300 µg/mL of Lesinurad and Allopurionol of the target concentration.

Keywords: RP-UPLC, Lesinurad, Allopurionol, Precision, Accuracy

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