The human origin recognition complex (ORC) is essential for pre-RC assembly, mitosis and maintenance of nuclear structure

1. Tiling_sgRNA_CRISPR_screen.tar.gz
   • ATCACG.fastq.gz    initial time point for HCT116 cell line Replicate1
   • TTAGGC.fastq.gz    initial time point for HCT116 cell line Replicate2
   • TGACCA.fastq.gz    final time point for HCT116 cell line Replicate1
   • GGCTAC.fastq.gz    final time point for HCT116 cell line Replicate2
   • CTTGTA.fastq.gz    initial time point for RPE1 cell line
   • GAGTGG.fastq.gz    final time point for RPE2 cell line
   • HCT116_rep1.csv - LFC values
   • HCT116_rep2.csv - LFC values
   • RPE1.csv - LFC values
   • ReadMe.txt file 
      
2. SMASH.zip
   • HCT116p53WT folder
     • HCT116p53WT_S6_R1_001.fastq.gz 
     • HCT116p53WT_S6_R2_001.fastq.gz
     • All other file with extensions .ps, .pdf, .txt are outputs of analysis using SMASH
   • HCT116p53null folder
     • HCT116p53null_S2_R1_001.fastq.gz
     • HCT116p53null_S2_R2_001.fastq.gz
     • All other file with extensions .ps, .pdf, .txt are outputs of analysis using SMASH
   • ORC1null folder
     • ORC1null_S3_R1_001.fastq.gz
     • ORC1null_S3_R2_001.fastq.gz
     • All other file with extensions .ps, .pdf, .txt are outputs of analysis using SMASH
   • ORC2null folder
     • ORC2null_S4_R1_001.fastq.gz
     • ORC2null_S4_R2_001.fastq.gz
     • All other file with extensions .ps, .pdf, .txt are outputs of analysis using SMASH
   • ReadMe.txt
      
3. ORC2null_ONT.zip
   • Alignments Folder - Alignment files
     • ORC2null.sort.bam.bai
     • ORC2null.sort.bam
   • SV_inference Folder - 
     • ORC2_CRISPR_sniffles.vcf - Analysis with Sniffles v1.0.11
     • ORC2_CRISPR_pbsv.vcf - Analysis with PBSV v2.2.0
   • ReadMe.txt

Funding provided by: National Cancer Institute
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000054
Award Number: P01-CA13106

Funding provided by: Cancer Center Support Grant*
Crossref Funder Registry ID:
Award Number: P50-CA045508