45607
doi
10.1007/s00259-016-3311-y
oai:zenodo.org:45607
user-inmind
user-eu
Anzilotti, S
IRCCS SDN, Via E. Gianturco 113, 80143, Naples, Italy
Coda, ARD
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy
Gramanzini, M
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy and Ceinge Biotecnologie Avanzate s. c. a r. l., Via G. Salvatore 486, 80145, Naples, Italy
Greco, A
Ceinge Biotecnologie Avanzate s. c. a r. l., Via G. Salvatore 486, 80145, Naples, Italy and Department of Advanced Biomedical Sciences, University "Federico II", Via S. Pansini 5, 80131, Naples, Italy
Panico, M
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy
Vinciguerra, A
Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of Medicine, University "Federico II", Via S. Pansini 5, 80131, Naples, Italy
Zannetti, A
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy
Vicidomini, C
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy
Dollé, F
CEA, Institute for Biomedical Imaging, 4 Place du Général Leclerc, 91401, Orsay, France
Pignataro, G
Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of Medicine, University "Federico II", Via S. Pansini 5, 80131, Naples, Italy
Quarantelli, M
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy
Annunziato, L
IRCCS SDN, Via E. Gianturco 113, 80143, Naples, Italy and Division of Pharmacology, Department of Neuroscience, Reproductive and Dentistry Sciences, School of Medicine, University "Federico II", Via S. Pansini 5, 80131, Naples, Italy
Brunetti, A
Ceinge Biotecnologie Avanzate s. c. a r. l., Via G. Salvatore 486, 80145, Naples, Italy and Department of Advanced Biomedical Sciences, University "Federico II", Via S. Pansini 5, 80131, Naples, Italy
Salvatore, M
IRCCS SDN, Via E. Gianturco 113, 80143, Naples, Italy
Pappatà, S
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy
Imaging of brain TSPO expression in a mouse model of amyotrophic lateral sclerosis with 18F-DPA-714 and micro-PET/CT.
Gargiulo, S
Institute of Biostructure and Bioimaging, National Research Council, Via T. De Amicis 95, 80145, Naples, Italy and Ceinge Biotecnologie Avanzate s. c. a r. l., Via G. Salvatore 486, 80145, Naples, Italy
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
SOD1G93A
TSPO
PET/CT
18F-DPA-714
mice
<p>PURPOSE: To evaluate the feasibility and sensitivity of <sup>18</sup>F-DPA-714 for the study of microglial activation in the brain and spinal cord of transgenic SOD1<sup>G93A</sup> mice using high-resolution PET/CT and to evaluate the Iba1 and TSPO expression with immunohistochemistry.</p>
<p>METHODS: Nine symptomatic SOD1<sup>G93A</sup> mice (aged 117 ± 12.7 days, clinical score range 1 - 4) and five WT SOD1 control mice (aged 108 ± 28.5 days) underwent <sup>18</sup>F-DPA-714 PET/CT. SUV ratios were calculated by normalizing the cerebellar (rCRB), brainstem (rBS), motor cortex (rMCX) and cervical spinal cord (rCSC) activities to that of the frontal association cortex. Two WT SOD1 and six symptomatic SOD1<sup>G93A</sup> mice were studied by immunohistochemistry.</p>
<p>RESULTS: In the symptomatic SOD1<sup>G93A</sup> mice, rCRB, rBS and rCSC were increased as compared to the values in WT SOD1 mice, with a statistically significantly difference in rBS (2.340 ± 0.784 vs 1.576 ± 0.287, p = 0.014). Immunofluorescence studies showed that TSPO expression was increased in the trigeminal, facial, ambiguus and hypoglossal nuclei, as well as in the spinal cord, of symptomatic SOD1<sup>G93A</sup> mice and was colocalized with increased Iba1 staining.</p>
<p>CONCLUSION: Increased <sup>18</sup>F-DPA-714 uptake can be detected with high-resolution PET/CT in the brainstem of transgenic SOD1<sup>G93A</sup> mice, a region known to be a site of degeneration and increased microglial activation in amyotrophic lateral sclerosis, in agreement with increased TSPO expression in the brainstem nuclei shown by immunostaining. Therefore, <sup>18</sup>F-DPA-714 PET/CT might be a suitable tool to evaluate microglial activation in the SOD1<sup>G93A</sup> mouse model.</p>
Zenodo
2016-01-27
info:eu-repo/semantics/article
629978
user-inmind
user-eu
award_title=Imaging of Neuroinflammation in Neurodegenerative Diseases; award_number=278850; award_identifiers_scheme=url; award_identifiers_identifier=https://cordis.europa.eu/projects/278850; funder_id=00k4n6c32; funder_name=European Commission;
1579539120.432609
5303972
md5:e6578f23bd4b0f2903e94ddcf1bc456a
https://zenodo.org/records/45607/files/Gargiulo_EurJNuclMedMolImaging_2016-P15-AAM.pdf
public
European Journal of Nuclear Medecine and Molecular Imaging
43
7
1348-59
2016-01-27