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Early detection of Cryphonectria parasitica in planting material (CPARA)

Barnes, Ann; Gottsberger, Richard; Persen, Ulrike; Leichtfried, Thomas; Heugens, Kurt; Douanla-Meli, Clovis; Simoni, Sauro; de Andrade, Eugenia


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    <subfield code="a">&lt;p&gt;&lt;em&gt;Cryphonectria parasitica&lt;/em&gt; is a devastating plant pathogen infecting chestnuts (&lt;em&gt;Castanea &lt;/em&gt;spp.). The long-distance spread of&amp;nbsp;&lt;em&gt;C. parasitica&lt;/em&gt;&amp;nbsp;is thought to occur through the movement of infected plants for planting, bark or wood material. There is evidence of a long latent period on infected plants. Long latent periods are problematic as affected trees do not show symptoms for some time and are being shipped to previously non-affected areas. Plants for planting were emphasized as the highest risk pathway for entry of the pathogen into protected zones, therefore a robust, sensitive detection method to screen for latent infections of planting material is required to support plant health inspection and potential certification regimes. This project was the result of a collaboration between specialists of &lt;em&gt;Cryphonectria parasitica&lt;/em&gt; disease in &lt;em&gt;Castanea &lt;/em&gt;spp. The CPARA project aimed to support the diagnosis of &lt;em&gt;C. parasitica&lt;/em&gt; in plants for planting, with a focus on latent infections. The project contributed to:&lt;/p&gt;

&lt;p&gt;- Development of a diagnostic protocol for the detection of &lt;em&gt;C. parasitica&lt;/em&gt; in planting material. This included development of an extraction protocol, and comparison of published real-time PCR tests.&lt;/p&gt;

&lt;p&gt;-&amp;nbsp;Validation of the published real-time PCR test by Chandelier &lt;em&gt;et al&lt;/em&gt;., (2019) for use on planting material to detect early infections before symptom expression.&amp;nbsp;&lt;/p&gt;</subfield>
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