Report Open Access

Early detection of Cryphonectria parasitica in planting material (CPARA)

Barnes, Ann; Gottsberger, Richard; Persen, Ulrike; Leichtfried, Thomas; Heugens, Kurt; Douanla-Meli, Clovis; Simoni, Sauro; de Andrade, Eugenia


JSON-LD (schema.org) Export

{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p><em>Cryphonectria parasitica</em> is a devastating plant pathogen infecting chestnuts (<em>Castanea </em>spp.). The long-distance spread of&nbsp;<em>C. parasitica</em>&nbsp;is thought to occur through the movement of infected plants for planting, bark or wood material. There is evidence of a long latent period on infected plants. Long latent periods are problematic as affected trees do not show symptoms for some time and are being shipped to previously non-affected areas. Plants for planting were emphasized as the highest risk pathway for entry of the pathogen into protected zones, therefore a robust, sensitive detection method to screen for latent infections of planting material is required to support plant health inspection and potential certification regimes. This project was the result of a collaboration between specialists of <em>Cryphonectria parasitica</em> disease in <em>Castanea </em>spp. The CPARA project aimed to support the diagnosis of <em>C. parasitica</em> in plants for planting, with a focus on latent infections. The project contributed to:</p>\n\n<p>- Development of a diagnostic protocol for the detection of <em>C. parasitica</em> in planting material. This included development of an extraction protocol, and comparison of published real-time PCR tests.</p>\n\n<p>-&nbsp;Validation of the published real-time PCR test by Chandelier <em>et al</em>., (2019) for use on planting material to detect early infections before symptom expression.&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Fera Science Ltd (Fera), Sand Hutton, United Kingdom", 
      "@type": "Person", 
      "name": "Barnes, Ann"
    }, 
    {
      "affiliation": "Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria", 
      "@type": "Person", 
      "name": "Gottsberger, Richard"
    }, 
    {
      "affiliation": "Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria", 
      "@type": "Person", 
      "name": "Persen, Ulrike"
    }, 
    {
      "affiliation": "Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria", 
      "@type": "Person", 
      "name": "Leichtfried, Thomas"
    }, 
    {
      "affiliation": "Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, Belgium", 
      "@type": "Person", 
      "name": "Heugens, Kurt"
    }, 
    {
      "affiliation": "Federal Ministry of Food and Agriculture (JKI), Braunschweig, Germany", 
      "@type": "Person", 
      "name": "Douanla-Meli, Clovis"
    }, 
    {
      "affiliation": "Council for Agricultural Research and Economics (CREA-DC), Firenze, Italy", 
      "@type": "Person", 
      "name": "Simoni, Sauro"
    }, 
    {
      "affiliation": "National Institute for Agricultural and Veterinarian Research (INIAV), Oeiras, Portugal", 
      "@type": "Person", 
      "name": "de Andrade, Eugenia"
    }
  ], 
  "headline": "Early detection of Cryphonectria parasitica in planting material (CPARA)", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2021-02-22", 
  "url": "https://zenodo.org/record/4555870", 
  "keywords": [
    "Euphresco, plant health, diagnostics, Cryphonectria parasitica, test performance study"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.4555870", 
  "@id": "https://doi.org/10.5281/zenodo.4555870", 
  "@type": "ScholarlyArticle", 
  "name": "Early detection of Cryphonectria parasitica in planting material (CPARA)"
}
346
223
views
downloads
All versions This version
Views 346346
Downloads 223223
Data volume 92.0 MB92.0 MB
Unique views 311311
Unique downloads 197197

Share

Cite as