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Early detection of Cryphonectria parasitica in planting material (CPARA)

Barnes, Ann; Gottsberger, Richard; Persen, Ulrike; Leichtfried, Thomas; Heugens, Kurt; Douanla-Meli, Clovis; Simoni, Sauro; de Andrade, Eugenia


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    "description": "<p><em>Cryphonectria parasitica</em> is a devastating plant pathogen infecting chestnuts (<em>Castanea </em>spp.). The long-distance spread of&nbsp;<em>C. parasitica</em>&nbsp;is thought to occur through the movement of infected plants for planting, bark or wood material. There is evidence of a long latent period on infected plants. Long latent periods are problematic as affected trees do not show symptoms for some time and are being shipped to previously non-affected areas. Plants for planting were emphasized as the highest risk pathway for entry of the pathogen into protected zones, therefore a robust, sensitive detection method to screen for latent infections of planting material is required to support plant health inspection and potential certification regimes. This project was the result of a collaboration between specialists of <em>Cryphonectria parasitica</em> disease in <em>Castanea </em>spp. The CPARA project aimed to support the diagnosis of <em>C. parasitica</em> in plants for planting, with a focus on latent infections. The project contributed to:</p>\n\n<p>- Development of a diagnostic protocol for the detection of <em>C. parasitica</em> in planting material. This included development of an extraction protocol, and comparison of published real-time PCR tests.</p>\n\n<p>-&nbsp;Validation of the published real-time PCR test by Chandelier <em>et al</em>., (2019) for use on planting material to detect early infections before symptom expression.&nbsp;</p>", 
    "language": "eng", 
    "title": "Early detection of Cryphonectria parasitica in planting material (CPARA)", 
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    "notes": "Report of the Euphresco project 2017-A-249 'Early detection of Cryphonectria parasitica in planting material (CPARA)'", 
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    "keywords": [
      "Euphresco, plant health, diagnostics, Cryphonectria parasitica, test performance study"
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    "publication_date": "2021-02-22", 
    "creators": [
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        "affiliation": "Fera Science Ltd (Fera), Sand Hutton, United Kingdom", 
        "name": "Barnes, Ann"
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        "affiliation": "Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria", 
        "name": "Gottsberger, Richard"
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      {
        "affiliation": "Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria", 
        "name": "Persen, Ulrike"
      }, 
      {
        "affiliation": "Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria", 
        "name": "Leichtfried, Thomas"
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      {
        "affiliation": "Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, Belgium", 
        "name": "Heugens, Kurt"
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      {
        "affiliation": "Federal Ministry of Food and Agriculture (JKI), Braunschweig, Germany", 
        "name": "Douanla-Meli, Clovis"
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      {
        "affiliation": "Council for Agricultural Research and Economics (CREA-DC), Firenze, Italy", 
        "name": "Simoni, Sauro"
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        "affiliation": "National Institute for Agricultural and Veterinarian Research (INIAV), Oeiras, Portugal", 
        "name": "de Andrade, Eugenia"
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