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Early detection of Cryphonectria parasitica in planting material (CPARA)

Barnes, Ann; Gottsberger, Richard; Persen, Ulrike; Leichtfried, Thomas; Heugens, Kurt; Douanla-Meli, Clovis; Simoni, Sauro; de Andrade, Eugenia


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  <identifier identifierType="DOI">10.5281/zenodo.4555870</identifier>
  <creators>
    <creator>
      <creatorName>Barnes, Ann</creatorName>
      <givenName>Ann</givenName>
      <familyName>Barnes</familyName>
      <affiliation>Fera Science Ltd (Fera), Sand Hutton, United Kingdom</affiliation>
    </creator>
    <creator>
      <creatorName>Gottsberger, Richard</creatorName>
      <givenName>Richard</givenName>
      <familyName>Gottsberger</familyName>
      <affiliation>Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria</affiliation>
    </creator>
    <creator>
      <creatorName>Persen, Ulrike</creatorName>
      <givenName>Ulrike</givenName>
      <familyName>Persen</familyName>
      <affiliation>Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria</affiliation>
    </creator>
    <creator>
      <creatorName>Leichtfried, Thomas</creatorName>
      <givenName>Thomas</givenName>
      <familyName>Leichtfried</familyName>
      <affiliation>Austrian Agency for Health and Food Safety, Austria (AGES), Vienna, Austria</affiliation>
    </creator>
    <creator>
      <creatorName>Heugens, Kurt</creatorName>
      <givenName>Kurt</givenName>
      <familyName>Heugens</familyName>
      <affiliation>Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Douanla-Meli, Clovis</creatorName>
      <givenName>Clovis</givenName>
      <familyName>Douanla-Meli</familyName>
      <affiliation>Federal Ministry of Food and Agriculture (JKI), Braunschweig, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>Simoni, Sauro</creatorName>
      <givenName>Sauro</givenName>
      <familyName>Simoni</familyName>
      <affiliation>Council for Agricultural Research and Economics (CREA-DC), Firenze, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>de Andrade, Eugenia</creatorName>
      <givenName>Eugenia</givenName>
      <familyName>de Andrade</familyName>
      <affiliation>National Institute for Agricultural and Veterinarian Research (INIAV), Oeiras, Portugal</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Early detection of Cryphonectria parasitica in planting material (CPARA)</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2021</publicationYear>
  <subjects>
    <subject>Euphresco, plant health, diagnostics, Cryphonectria parasitica, test performance study</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2021-02-22</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Report"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4555870</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.4555869</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;em&gt;Cryphonectria parasitica&lt;/em&gt; is a devastating plant pathogen infecting chestnuts (&lt;em&gt;Castanea &lt;/em&gt;spp.). The long-distance spread of&amp;nbsp;&lt;em&gt;C. parasitica&lt;/em&gt;&amp;nbsp;is thought to occur through the movement of infected plants for planting, bark or wood material. There is evidence of a long latent period on infected plants. Long latent periods are problematic as affected trees do not show symptoms for some time and are being shipped to previously non-affected areas. Plants for planting were emphasized as the highest risk pathway for entry of the pathogen into protected zones, therefore a robust, sensitive detection method to screen for latent infections of planting material is required to support plant health inspection and potential certification regimes. This project was the result of a collaboration between specialists of &lt;em&gt;Cryphonectria parasitica&lt;/em&gt; disease in &lt;em&gt;Castanea &lt;/em&gt;spp. The CPARA project aimed to support the diagnosis of &lt;em&gt;C. parasitica&lt;/em&gt; in plants for planting, with a focus on latent infections. The project contributed to:&lt;/p&gt;

&lt;p&gt;- Development of a diagnostic protocol for the detection of &lt;em&gt;C. parasitica&lt;/em&gt; in planting material. This included development of an extraction protocol, and comparison of published real-time PCR tests.&lt;/p&gt;

&lt;p&gt;-&amp;nbsp;Validation of the published real-time PCR test by Chandelier &lt;em&gt;et al&lt;/em&gt;., (2019) for use on planting material to detect early infections before symptom expression.&amp;nbsp;&lt;/p&gt;</description>
    <description descriptionType="Other">Report of the Euphresco project 2017-A-249 'Early detection of Cryphonectria parasitica in planting material (CPARA)'</description>
  </descriptions>
</resource>
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