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Early detection of Cryphonectria parasitica in planting material (CPARA)

Barnes, Ann; Gottsberger, Richard; Persen, Ulrike; Leichtfried, Thomas; Heugens, Kurt; Douanla-Meli, Clovis; Simoni, Sauro; de Andrade, Eugenia


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.4555870", 
  "language": "eng", 
  "title": "Early detection of Cryphonectria parasitica in planting material (CPARA)", 
  "issued": {
    "date-parts": [
      [
        2021, 
        2, 
        22
      ]
    ]
  }, 
  "abstract": "<p><em>Cryphonectria parasitica</em> is a devastating plant pathogen infecting chestnuts (<em>Castanea </em>spp.). The long-distance spread of&nbsp;<em>C. parasitica</em>&nbsp;is thought to occur through the movement of infected plants for planting, bark or wood material. There is evidence of a long latent period on infected plants. Long latent periods are problematic as affected trees do not show symptoms for some time and are being shipped to previously non-affected areas. Plants for planting were emphasized as the highest risk pathway for entry of the pathogen into protected zones, therefore a robust, sensitive detection method to screen for latent infections of planting material is required to support plant health inspection and potential certification regimes. This project was the result of a collaboration between specialists of <em>Cryphonectria parasitica</em> disease in <em>Castanea </em>spp. The CPARA project aimed to support the diagnosis of <em>C. parasitica</em> in plants for planting, with a focus on latent infections. The project contributed to:</p>\n\n<p>- Development of a diagnostic protocol for the detection of <em>C. parasitica</em> in planting material. This included development of an extraction protocol, and comparison of published real-time PCR tests.</p>\n\n<p>-&nbsp;Validation of the published real-time PCR test by Chandelier <em>et al</em>., (2019) for use on planting material to detect early infections before symptom expression.&nbsp;</p>", 
  "author": [
    {
      "family": "Barnes, Ann"
    }, 
    {
      "family": "Gottsberger, Richard"
    }, 
    {
      "family": "Persen, Ulrike"
    }, 
    {
      "family": "Leichtfried, Thomas"
    }, 
    {
      "family": "Heugens, Kurt"
    }, 
    {
      "family": "Douanla-Meli, Clovis"
    }, 
    {
      "family": "Simoni, Sauro"
    }, 
    {
      "family": "de Andrade, Eugenia"
    }
  ], 
  "note": "Report of the Euphresco project 2017-A-249 'Early detection of Cryphonectria parasitica in planting material (CPARA)'", 
  "type": "article", 
  "id": "4555870"
}
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