Three new species of Nothopodinae (Acari: Eriophyidae) from China

ABSTRACT Three new vagrant species of the subfamily Nothopodinae from China are described and illustrated. These species are Kuangella trifoliatae sp. nov. collected from Derris trifoliata Lour. (Leguminosae), Neodisella garciniae sp. nov. collected from Garcinia oblongifolia Champ. ex Benth. (Clusiaceae), and Nonthaburinus roxburghianae sp. nov. found on Engelhardtia roxburghiana Lindl. (Juglandaceae). http://www.zoobank.org/urn:lsid:zoobank.org:pub:3C92A4AC-8E1F-49CC-9035-B1093D207A99


Introduction
Eriophyoid mites are tiny obligate phytophagous species (Lindquist and Oldfield 1996). Most of them are highly specialized herbivores forming galls or living freely on a single plant species or genus (Oldfield 1996;Skoracka et al. 2010). Derris trifoliata Lour. (Leguminosae) is a poisonous vine, used extensively for insect pests control and distributed in the Asia tropical regions (Witt et al. 1999). Two eriophyoid mites have been found on Derris spp. in China: Abacarus ellipticae Huang, 2001a described from D. elliptica Benth. and Tetra guangxiensis , described from Derris sp. (Huang 2001a;Li et al. 2009). Garcinia (Clusiaceae) is thrives in tropical habitats, including tropical Asia, tropical America, and southern Africa. It comprises about 450 species and is currently gaining much medicinal and culinary importance (Yapwattanaphun et al. 2000;Li et al. 2007). Among the Garcinia species, G. oblongifolia Champ. ex Benth. is mediumsized shrub and widely distributed in southern China and northern Vietnam (Shan et al. 2012). The current literature contains records for two eriophyoid species from Garcinia in China: Mediugum sanasaii Huang, 2001 from G. subelliptica Merr., and Phyllocoptruta garcinia Wei & Xie, 2009 from G. paucinervis Chun & How (Huang 2001b;Wei et al. 2009). So far, Diptilomiopus engelhardter Xue, Chen & Hong 2013 is the only eriophyoid mite species reported from Engelhardtia roxburghiana Lindl. (Juglandaceae), which is an evergreen to briefly deciduous tree and widely available in China, Vietnam, Thailand, Burma, and India (Yang and Lu 1996;Xue et al. 2013). Its leaves have been traditionally consumed as sweet tea for possible body weight control.
The subfamily Nothopodinae belongs to the family Eriophyidae and is established by Keifer (1956). It can be distinguished from other subfamilies by the tibiae reduced or completely fused with tarsi, and without seta (Amrine et al. 2003). Seventeen genera and 82 species of the subfamily Nothopodinae are found in China Hong et al. 2010;Li et al. 2010;Zhang et al. 2011;Wang et al. 2013). This article provides the descriptions of three new Nothopodinae species which were found new to science.

Materials and methods
Eriophyoid mites were found and collected from the undersurfaces of leaves of the plants Derris trifoliata Lour. (Figure 1(a)), Garcinia oblongifolia Champ. ex Benth. (Figure 1(b)), and Engelhardtia roxburghiana Lindl. (Figure 1(c)) with the aid of a 60× hand magnifying glass in Hainan island and stored in a sucrose-ethanol solution (75% ethanol).
In the laboratory, mites were cleared in Nesbitt's solution at room temperature and mounted in Heinze's medium on glass microscope slides according to Kuang protocol (Kuang 1986). All specimens were examined with the aid of an Olympus CX41 microscope under phase contrast (Plan achromatic objectives: ×4/0.1, ×10/0.25, ×40/0.65; ×100/1.25 oil immersion; wide field eyepiece 15×). Micrographs were obtained by Nikon DS-Ri2 microscope. Schematic drawings were based on de Lillo et al. (2010). Measurements and morphological terminology follow that of de Lillo et al. (2010) and measurement units are in micrometres (μm) and are rounded off to the nearest full number. All are lengths if not otherwise specified. The number of measured specimens is given in parentheses at the beginning of each description. The measurement of the holotype is followed by range of the paratypes measurements in parentheses. The count of ventral annuli starts from the first full semi-annulus behind coxisternal plates; the length of each leg is measured from the trochanteral base to the tip of the tarsus, excluding the empodium and solenidion (ω).

Relation to host
The mites are vagrants on the undersurfaces of leaves, with no visible damage.

Etymology
The specific designation trifoliatae is derived from the specific name of the type host plant, trifoliata.

Relation to host
The mites are vagrants on the undersurfaces of leaves, with no visible damage.

Etymology
The specific designation garciniae is derived from the generic name of the type host plant, garcinia.

Note
Only one species of the genus Nonthaburinus namely N. litchi Chandrapatya, 1996, found on Litchi chinensis Sonn. (Sapindaceae) from Thailand. In the current paper the second species, N. roxburghianae sp. nov. found on Engelhardtia roxburghiana Lindl. (Juglandaceae) in China is described.

Relation to host
The mites are vagrants on the undersurfaces of leaves, with no visible damage.

Etymology
The specific designation roxburghianae is derived from the specific name of the type host plant, roxburghiana.