Journal article Open Access

Comparative study of autofluorescence in flat and tapered optical fibers towards application in depth-resolved fluorescence lifetime photometry in brain tissue

Bianco, Marco; Balena, Antonio; Pisanello, Marco; Pisano, Filippo; Sileo, Leonardo; Spagnolo, Barbara; Montinaro, Cinzia; Sabatini, Bernardo L.; De Vittorio, Massimo; Pisanello, Ferruccio


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  <identifier identifierType="URL">https://zenodo.org/record/4501735</identifier>
  <creators>
    <creator>
      <creatorName>Bianco, Marco</creatorName>
      <givenName>Marco</givenName>
      <familyName>Bianco</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-9693-1106</nameIdentifier>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Via per Monteroni, 73100 Lecce, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Balena, Antonio</creatorName>
      <givenName>Antonio</givenName>
      <familyName>Balena</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-4314-4314</nameIdentifier>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Via per Monteroni, 73100 Lecce, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Pisanello, Marco</creatorName>
      <givenName>Marco</givenName>
      <familyName>Pisanello</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-2179-7883</nameIdentifier>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Pisano, Filippo</creatorName>
      <givenName>Filippo</givenName>
      <familyName>Pisano</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-5499-160X</nameIdentifier>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Sileo, Leonardo</creatorName>
      <givenName>Leonardo</givenName>
      <familyName>Sileo</familyName>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Spagnolo, Barbara</creatorName>
      <givenName>Barbara</givenName>
      <familyName>Spagnolo</familyName>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Montinaro, Cinzia</creatorName>
      <givenName>Cinzia</givenName>
      <familyName>Montinaro</familyName>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy; Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università del Salento, Via per Monteroni, 73100 Lecce, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Sabatini, Bernardo L.</creatorName>
      <givenName>Bernardo L.</givenName>
      <familyName>Sabatini</familyName>
      <affiliation>Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA</affiliation>
    </creator>
    <creator>
      <creatorName>De Vittorio, Massimo</creatorName>
      <givenName>Massimo</givenName>
      <familyName>De Vittorio</familyName>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Via per Monteroni, 73100 Lecce, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Pisanello, Ferruccio</creatorName>
      <givenName>Ferruccio</givenName>
      <familyName>Pisanello</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-1489-7758</nameIdentifier>
      <affiliation>Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies, Via Barsanti 14, 73010 Arnesano (Lecce), Italy</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Comparative study of autofluorescence in flat and tapered optical fibers towards application in depth-resolved fluorescence lifetime photometry in brain tissue</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-26</date>
  </dates>
  <resourceType resourceTypeGeneral="JournalArticle"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4501735</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1364/BOE.410244</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;Abstract:&lt;/strong&gt; As the scientific community seeks efficient optical neural interfaces with sub-cortical structures of the mouse brain, a wide set of technologies and methods is being developed to monitor cellular events through fluorescence signals generated by genetically encoded molecules. Among these technologies, tapered optical fibers (TFs) take advantage of the modal properties of narrowing waveguides to enable both depth-resolved and wide-volume light collection from scattering tissue, with minimized invasiveness with respect to standard flat fiber stubs (FFs). However, light guided in patch cords as well as in FFs and TFs can result in autofluorescence (AF) signal, which can act as a source of time-variable noise and limit their application to probe fluorescence lifetime in vivo. In this work, we compare the AF signal of FFs and TFs, highlighting the influence of the cladding composition on AF generation. We show that the autofluorescence signal generated in TFs has a peculiar coupling pattern with guided modes, and that far-field detection can be exploited to separate functional fluorescence from AF. On these bases, we provide evidence that TFs can be employed to implement depth-resolved fluorescence lifetime photometry, potentially enabling the extraction of a new set of information from deep brain regions, as time-correlating single photon counting starts to be applied in freely-moving animals to monitor the intracellular biochemical state of neurons.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/677683/">677683</awardNumber>
      <awardTitle>Multipoint Optical DEvices for Minimally invasive neural circuits interface</awardTitle>
    </fundingReference>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/692943/">692943</awardNumber>
      <awardTitle>All-optical brain-to-brain behaviour and information transfer</awardTitle>
    </fundingReference>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/828972/">828972</awardNumber>
      <awardTitle>BRInGing nano-pHoTonics into the brain</awardTitle>
    </fundingReference>
  </fundingReferences>
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