January 20, 2021 Technical note Open Access

Lepine, Paula; Tabatabaei, Mahdieh; Da Silva, Ronan; Durcan, Thomas M.

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  <identifier identifierType="DOI">10.5281/zenodo.4451592</identifier>
  <creators>
    <creator>
      <creatorName>Lepine, Paula</creatorName>
      <givenName>Paula</givenName>
      <familyName>Lepine</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Tabatabaei, Mahdieh</creatorName>
      <givenName>Mahdieh</givenName>
      <familyName>Tabatabaei</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Da Silva, Ronan</creatorName>
      <givenName>Ronan</givenName>
      <familyName>Da Silva</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
    <creator>
      <creatorName>Durcan, Thomas M.</creatorName>
      <givenName>Thomas M.</givenName>
      <familyName>Durcan</familyName>
      <affiliation>MNI, McGill University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Cryosectioning of fixed and frozen tissue</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2021</publicationYear>
  <subjects>
    <subject>cryosectioning</subject>
    <subject>tissue</subject>
    <subject>OCT embedding</subject>
    <subject>Cryostat</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2021-01-20</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Technical note</resourceType>
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  <version>V1.0</version>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
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  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;This protocol will provide a standardized procedure on methods developed for cryosectioning of fixed or fresh frozen tissue in Optimal Cutting Temperature (OCT) using a motorized cryostat. This SOP also outlines minimum assessment criteria that should be in place to evaluate the quality and integrity of frozen tissue sections and troubleshooting steps when issues with sectioning arise.&lt;/p&gt;</description>
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