10.3390/jof7010039
https://zenodo.org/records/4441893
oai:zenodo.org:4441893
Daou, Mariane
Mariane
Daou
0000-0002-8651-965X
Farfan Soto, Clementina
Clementina
Farfan Soto
Majira, Amel
Amel
Majira
Cézard, Laurent
Laurent
Cézard
Cottyn, Betty
Betty
Cottyn
Pion, Florian
Florian
Pion
Navarro, David
David
Navarro
Correia, Lydia Oliveira
Lydia Oliveira
Correia
0000-0002-8590-250X
Drula, Elodie
Elodie
Drula
Record, Eric
Eric
Record
0000-0002-7545-9997
Raouche, Sana
Sana
Raouche
0000-0001-5937-4902
Baumberger, Stéphanie
Stéphanie
Baumberger
0000-0002-9550-4935
Faulds, Craig B.
Craig B.
Faulds
Fungal Treatment for the Valorization of Technical Soda Lignin
Zenodo
2021
filamentous fungi
technical lignin
oxidoreductases
secretomic analysis
Polyporus brumalis
Pycnoporus sanguineus
Leiotrametes menziesii
2021-01-09
eng
https://zenodo.org/communities/720303
https://zenodo.org/communities/eu
Creative Commons Attribution 4.0 International
Technical lignins produced as a by-product in biorefinery processes represent a potential source of renewable carbon. In consideration of the possibilities of the industrial transformation of this substrate into various valuable bio-based molecules, the biological deconstruction of a technical soda lignin by filamentous fungi was investigated. The ability of three basidiomycetes (Polyporus brumalis, Pycnoporus sanguineus and Leiotrametes menziesii) to modify this material, the resultant structural and chemical changes, and the secreted proteins during growth on this substrate were investigated. The three fungi could grow on the technical lignin alone, and the growth rate increased when the media were supplemented with glucose or maltose. The proteomic analysis of the culture supernatants after three days of growth revealed the secretion of numerous Carbohydrate-Active Enzymes (CAZymes). The secretomic profiles varied widely between the strains and the presence of technical lignin alone triggered the early secretion of many lignin-acting oxidoreductases. The secretomes were notably rich in glycoside hydrolases and H2O2-producing auxiliary activity enzymes with copper radical oxidases being induced on lignin for all strains. The lignin treatment by fungi modified both the soluble and insoluble lignin fractions. A significant decrease in the amount of soluble higher molar mass compounds was observed in the case of P. sanguineus. This strain was also responsible for the modification of the lower molar mass compounds of the lignin insoluble fraction and a 40% decrease in the thioacidolysis yield. The similarity in the activities of P. sanguineus and P. brumalis in modifying the functional groups of the technical lignin were observed, the results suggest that the lignin has undergone structural changes, or at least changes in its composition, and pave the route for the utilization of filamentous fungi to functionalize technical lignins and produce the enzymes of interest for biorefinery applications.
European Commission
10.13039/501100000780
720303
Zero Waste Ligno-Cellulosic Biorefineries by Integrated Lignin Valorisation