Matteo Gasbarri
Philip V'kovski
Giulia Torriani
Volker Thiel
Francesco Stellacci
Caroline Tapparel
Valeria Cagno
2020-11-30
<p>Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) depends on angiotensin converting enzyme 2 (ACE2) for cellular entry, but it might also rely on attachment receptors such as heparan sulfates. Several groups have recently demonstrated an affinity of the SARS-CoV2 spike protein for heparan sulfates and a reduced binding to cells in the presence of heparin or heparinase treatment. Here, we investigated the inhibitory activity of several sulfated and sulfonated molecules, which prevent interaction with heparan sulfates, against vesicular stomatitis virus (VSV)-pseudotyped-SARS-CoV-2 and the authentic SARS-CoV-2. Sulfonated cyclodextrins and nanoparticles that have recently shown broad-spectrum non-toxic virucidal activity against many heparan sulfates binding viruses showed inhibitory activity in the micromolar and nanomolar ranges, respectively. In stark contrast with the mechanisms that these compounds present for these other viruses, the inhibition against SARS-CoV-2 was found to be simply reversible.</p>
https://doi.org/10.5281/zenodo.4321274
oai:zenodo.org:4321274
Zenodo
https://doi.org/10.5281/zenodo.4321273
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Microorganism, 8, 1894, (2020-11-30)
SARS-CoV-2; antiviral; heparan sulfates; attachment inhibitor
SARS-CoV-2 Inhibition by Sulfonated Compounds
info:eu-repo/semantics/other