Journal article Open Access

Fagopyrum esculentum ssp. ancestrale-A Hybrid Species Between Diploid F. cymosum and F. esculentum

Cheng Cheng; Yu Fan; Yu Tang; Kaixuan Zhang; Dinesh C. Joshi; Rintu Jha; Dagmar Janovska; Vladimir Meglič; Mingli Yan; Meiliang Zhou


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  <identifier identifierType="URL">https://zenodo.org/record/4315238</identifier>
  <creators>
    <creator>
      <creatorName>Cheng Cheng</creatorName>
    </creator>
    <creator>
      <creatorName>Yu Fan</creatorName>
    </creator>
    <creator>
      <creatorName>Yu Tang</creatorName>
    </creator>
    <creator>
      <creatorName>Kaixuan Zhang</creatorName>
    </creator>
    <creator>
      <creatorName>Dinesh C. Joshi</creatorName>
    </creator>
    <creator>
      <creatorName>Rintu Jha</creatorName>
    </creator>
    <creator>
      <creatorName>Dagmar Janovska</creatorName>
    </creator>
    <creator>
      <creatorName>Vladimir Meglič</creatorName>
    </creator>
    <creator>
      <creatorName>Mingli Yan</creatorName>
    </creator>
    <creator>
      <creatorName>Meiliang Zhou</creatorName>
    </creator>
  </creators>
  <titles>
    <title>Fagopyrum esculentum ssp. ancestrale-A Hybrid Species Between Diploid F. cymosum and F. esculentum</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <dates>
    <date dateType="Issued">2020-07-16</date>
  </dates>
  <resourceType resourceTypeGeneral="JournalArticle"/>
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    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4315238</alternateIdentifier>
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  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3389/fpls.2020.01073</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/ecobreed</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;em&gt;Fagopyrum cymosum&lt;/em&gt;&amp;nbsp;is considered as most probable wild ancestor of cultivated buckwheat. However, the evolutionary route from&amp;nbsp;&lt;em&gt;F. cymosum&lt;/em&gt;&amp;nbsp;to&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;&amp;nbsp;remains to be deciphered. We hypothesized that a hybrid species exists in natural habitats between diploid&amp;nbsp;&lt;em&gt;F. cymosum&lt;/em&gt;&amp;nbsp;and&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;. The aim of this research was to determine the phylogenetic position of&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;&amp;nbsp;ssp.&amp;nbsp;&lt;em&gt;ancestrale&lt;/em&gt;&amp;nbsp;and to provide new thoughts on buckwheat evolution. Different methodologies including evaluation of morphological traits, determination of secondary metabolites, fluorescence&amp;nbsp;&lt;em&gt;in situ&lt;/em&gt;&amp;nbsp;hybridization (FISH), comparative chloroplast genomics, and molecular markers were deployed to determine the phylogenetic relationship of&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;&amp;nbsp;ssp.&amp;nbsp;&lt;em&gt;ancestrale&lt;/em&gt;&amp;nbsp;with&amp;nbsp;&lt;em&gt;F. cymosum&lt;/em&gt;&amp;nbsp;and&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;. The ambiguity observed in morphological pattern of genetic variation in three species revealed that&amp;nbsp;&lt;em&gt;F.&lt;/em&gt;&amp;nbsp;&lt;em&gt;esculentum&lt;/em&gt;&amp;nbsp;ssp. ancestrale is closely related to&amp;nbsp;&lt;em&gt;F. cymosum&lt;/em&gt;&amp;nbsp;and&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;. Flavonoid analysis revealed that&amp;nbsp;&lt;em&gt;F.&lt;/em&gt;&amp;nbsp;&lt;em&gt;esculentum&lt;/em&gt;&amp;nbsp;ssp.&amp;nbsp;&lt;em&gt;ancestrale&lt;/em&gt;&amp;nbsp;is closely related to&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;. Comparative chloroplast genome analysis further supported the close proximity of&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;&amp;nbsp;ssp.&amp;nbsp;&lt;em&gt;ancestrale&lt;/em&gt;&amp;nbsp;with&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;. Additionally, molecular marker analysis revealed that&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;&amp;nbsp;ssp.&amp;nbsp;&lt;em&gt;ancestrale&lt;/em&gt;&amp;nbsp;exhibits co-dominance with the bands amplified by&amp;nbsp;&lt;em&gt;F. cymosum&lt;/em&gt;&amp;nbsp;and&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;. These finding provided supporting evidence in favor of the hypothesis that&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;&amp;nbsp;ssp.&amp;nbsp;&lt;em&gt;ancestrale&lt;/em&gt;&amp;nbsp;is a hybrid species between&amp;nbsp;&lt;em&gt;F. cymosum&lt;/em&gt;&amp;nbsp;to&amp;nbsp;&lt;em&gt;F. esculentum&lt;/em&gt;, which was probably originated by spontaneous hybridization under natural conditions.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/100010661</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/771367/">771367</awardNumber>
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