Sapoznik, Etai
Chang, Bo-Jui
Huh, Jaewon
Ju, Robert
Azarova, Evgenia V.
Pohlkamp, Theresa
Welf, Erik S.
Broadbent, David
Carisey, Alexandre F.
Stehbens, Samantha J.
Lee, Kyung-Min
Marin, Arnoldo
Hanker, Ariella B.
Schmidt, Jens C.
Arteaga, Carlos L.
Yang, Bin
Koboyashi, Yoshihiko
Tata, Purushothama R.
Kruithoff, Rory
Doubrovinski, Konstantin
Shepherd, Doug P.
York, Andrew G.
Millet-Sikking, Alfred
Dean, Kevin M.
Fiolka, Reto P.
2020-11-10
<p>We present an Oblique Plane Microscope that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of Lattice Light-Sheet Microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.</p>
https://doi.org/10.5281/zenodo.4266823
oai:zenodo.org:4266823
Zenodo
https://doi.org/10.7554/eLife.57681
https://doi.org/10.5281/zenodo.4266822
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
eLife, (2020-11-10)
Oblique plane microscopy
Light-sheet microscopy
Selective plane illumination microscopy
A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics - Public Data
info:eu-repo/semantics/article