10.5281/zenodo.4266823
https://zenodo.org/records/4266823
oai:zenodo.org:4266823
Sapoznik, Etai
Etai
Sapoznik
0000-0001-8472-0299
University of Texas Southwestern Medical Center
Chang, Bo-Jui
Bo-Jui
Chang
0000-0002-5513-7106
University of Texas Southwestern Medical Center
Huh, Jaewon
Jaewon
Huh
0000-0003-3954-8092
University of Texas Southwestern Medical Center
Ju, Robert
Robert
Ju
0000-0002-9850-9803
University of Queensland
Azarova, Evgenia V.
Evgenia V.
Azarova
University of Texas Southwestern Medical Center
Pohlkamp, Theresa
Theresa
Pohlkamp
0000-0003-3923-1917
University of Texas Southwestern Medical Center
Welf, Erik S.
Erik S.
Welf
0000-0002-5968-3323
University of Texas Southwestern Medical Center
Broadbent, David
David
Broadbent
Michigan State University
Carisey, Alexandre F.
Alexandre F.
Carisey
0000-0003-1326-2205
Baylor College of Medicine and Texas Children's Hospital
Stehbens, Samantha J.
Samantha J.
Stehbens
0000-0002-8145-2708
University of Queensland
Lee, Kyung-Min
Kyung-Min
Lee
0000-0001-8381-2423
University of Texas Southwestern Medical Center
Marin, Arnoldo
Arnoldo
Marin
University of Texas Southwestern Medical Center
Hanker, Ariella B.
Ariella B.
Hanker
0000-0002-8655-8341
University of Texas Southwestern Medical Center
Schmidt, Jens C.
Jens C.
Schmidt
0000-0001-9061-7853
Michigan State University
Arteaga, Carlos L.
Carlos L.
Arteaga
0000-0003-0870-9586
University of Texas Southwestern Medical Center
Yang, Bin
Bin
Yang
0000-0001-5138-4663
Chan Zuckerberg Biohub
Koboyashi, Yoshihiko
Yoshihiko
Koboyashi
Duke University School of Medicine
Tata, Purushothama R.
Purushothama R.
Tata
Duke University School of Medicine
Kruithoff, Rory
Rory
Kruithoff
Arizona State University
Doubrovinski, Konstantin
Konstantin
Doubrovinski
0000-0002-4403-948X
University of Texas Southwestern Medical Center
Shepherd, Doug P.
Doug P.
Shepherd
0000-0001-9087-0832
Arizona State University
York, Andrew G.
Andrew G.
York
Calico Life Sciences LLC
Millet-Sikking, Alfred
Alfred
Millet-Sikking
Calico Life Sciences LLC
Dean, Kevin M.
Kevin M.
Dean
0000-0003-0839-2320
University of Texas Southwestern Medical Center
Fiolka, Reto P.
Reto P.
Fiolka
0000-0002-4636-5000
University of Texas Southwestern Medical Center
A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics - Public Data
Zenodo
2020
Oblique plane microscopy
Light-sheet microscopy
Selective plane illumination microscopy
2020-11-10
10.7554/eLife.57681
10.5281/zenodo.4266822
1
Creative Commons Attribution 4.0 International
We present an Oblique Plane Microscope that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of Lattice Light-Sheet Microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.