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Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence

Tjou-Tam-Sin, Napoleon; Vogelaar, Martijn; Li, XIang; Čermák, Vaclav; Fornefeld, Eva; van der Wolf, Jean Martin; Valentini, Franco; Fraser, Karen; Cara, Magdalena; Yildiz, Nilufer; Yuzbasioglu, Eda; Karahan, Aynur; Ustun, Nursen; Kreuze, Jan


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    "description": "<p>The EU directive 2006/63/EC describes a detailed protocol for the official testing of <em>Ralstonia solanacearum</em>, that is internationally recognized and has been implemented in many diagnostic laboratories across Europe and beyond. In this protocol, the confirmation of the identity of the bacterium is performed by a laborious, time-consuming and expensive pathogenicity test. Additionally, the described protocols involved in the official testing of <em>Ralstonia solanacearum</em> (for detection and/or identification) require a lot of time, a high level of quarantine measures, and a high degree of expertise. The aim of this project was to develop, and evaluate molecular diagnostic methods for the detection and identification of <em>Ralstonia solanacearum</em> and verification of its virulence that would be faster, more specific and robust. Based on the current taxonomic insights, the high degree of heterogenity present inside the <em>Ralstonia solanacearum</em> species complex (RSSC) has resulted in the clear distinction of three new species inside this complex; the requirement defined in this Euphresco project was that the new molecular tests could be optimally implemented for the detection and identification of <em>Ralstonia solanacearum</em> and <em>Ralstonia pseudosolanacearu</em>m, but not of <em>Ralstonia syzygii</em>.</p>\n\n<p>The two main objectives of the project were:</p>\n\n<p>1) To identify <em>Ralstonia solanacearum </em>virulence genes, and subsequently develop a PCR test on those virulence genes. The development of such a test could substitute the pathogenicity test required by EU directive 2006/63/EC for complete diagnosis of <em>Ralstonia solanacearum</em>.</p>\n\n<p>2) To verify other molecular methods to detect or identify <em>Ralstonia solanacearum </em>strains. These methods include the real-time Loop-mediated isothermal amplication test (LAMP) and the recombinase-polymerase amplification assay (RPA).</p>", 
    "language": "eng", 
    "title": "Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence", 
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    "notes": "Report of the Euphresco project 2016-A-180 'Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence'", 
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    "keywords": [
      "Euphresco, plant health, diagnostics, Ralstonia solanacearum, Ralstonia pseudosolanacearum, virulence genes, IFAS, real-time PCR, real-time LAMP"
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    "publication_date": "2020-10-29", 
    "creators": [
      {
        "affiliation": "National Reference Center, National Plant Protection Organisaton (NVWA), Wageningen, The Netherlands", 
        "name": "Tjou-Tam-Sin, Napoleon"
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      {
        "affiliation": "National Reference Center, National Plant Protection Organisaton (NVWA), Wageningen, The Netherlands", 
        "name": "Vogelaar, Martijn"
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      {
        "affiliation": "Canadian Food Inspection Agency (CFIA), Charlottetown, Canada", 
        "name": "Li, XIang"
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        "affiliation": "Central Institute for Supervising and Testing in Agriculture (\u00daKZ\u00dcZ), Olomouc, Czech Republic", 
        "name": "\u010cerm\u00e1k, Vaclav"
      }, 
      {
        "affiliation": "Julius K\u00fchn-Institute, Federal Research Centre for Cultivated Plants (JKI), Kleinmachnow, Germany", 
        "name": "Fornefeld, Eva"
      }, 
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        "affiliation": "Wageningen Plant Research (WUR), Wageningen, the Netherlands", 
        "name": "van der Wolf, Jean Martin"
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        "affiliation": "International Center for Advanced Mediterranean Agronomic Studies (CIHEAM-IAMB), Bari, Italy", 
        "name": "Valentini, Franco"
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        "affiliation": "Science and Advice for Scottish Agriculture (SASA), Edinburgh, United Kingdom", 
        "name": "Fraser, Karen"
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        "affiliation": "Agricultural University of Tirana (UBT), Tirana, Albania", 
        "name": "Cara, Magdalena"
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        "affiliation": "Biological Control Research Institute (BCRI), Adana, Turkey", 
        "name": "Yildiz, Nilufer"
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        "affiliation": "Biological Control Research Institute (BCRI), Adana, Turkey", 
        "name": "Yuzbasioglu, Eda"
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        "affiliation": "Plant Protection Central Research Institute, Ankara (PPCRI), Ankara, Turkey", 
        "name": "Karahan, Aynur"
      }, 
      {
        "affiliation": "Plant Protection Research Institute, Bornova (PPRI), Izmir, Turkey", 
        "name": "Ustun, Nursen"
      }, 
      {
        "affiliation": "International Potato Center (CIP), Lima, Peru", 
        "name": "Kreuze, Jan"
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