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Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence

Tjou-Tam-Sin, Napoleon; Vogelaar, Martijn; Li, XIang; Čermák, Vaclav; Fornefeld, Eva; van der Wolf, Jean Martin; Valentini, Franco; Fraser, Karen; Cara, Magdalena; Yildiz, Nilufer; Yuzbasioglu, Eda; Karahan, Aynur; Ustun, Nursen; Kreuze, Jan


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  <identifier identifierType="DOI">10.5281/zenodo.4153357</identifier>
  <creators>
    <creator>
      <creatorName>Tjou-Tam-Sin, Napoleon</creatorName>
      <givenName>Napoleon</givenName>
      <familyName>Tjou-Tam-Sin</familyName>
      <affiliation>National Reference Center, National Plant Protection Organisaton (NVWA), Wageningen, The Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Vogelaar, Martijn</creatorName>
      <givenName>Martijn</givenName>
      <familyName>Vogelaar</familyName>
      <affiliation>National Reference Center, National Plant Protection Organisaton (NVWA), Wageningen, The Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Li, XIang</creatorName>
      <givenName>XIang</givenName>
      <familyName>Li</familyName>
      <affiliation>Canadian Food Inspection Agency (CFIA), Charlottetown, Canada</affiliation>
    </creator>
    <creator>
      <creatorName>Čermák, Vaclav</creatorName>
      <givenName>Vaclav</givenName>
      <familyName>Čermák</familyName>
      <affiliation>Central Institute for Supervising and Testing in Agriculture (ÚKZÜZ), Olomouc, Czech Republic</affiliation>
    </creator>
    <creator>
      <creatorName>Fornefeld, Eva</creatorName>
      <givenName>Eva</givenName>
      <familyName>Fornefeld</familyName>
      <affiliation>Julius Kühn-Institute, Federal Research Centre for Cultivated Plants (JKI), Kleinmachnow, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>van der Wolf, Jean Martin</creatorName>
      <givenName>Jean Martin</givenName>
      <familyName>van der Wolf</familyName>
      <affiliation>Wageningen Plant Research (WUR), Wageningen, the Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Valentini, Franco</creatorName>
      <givenName>Franco</givenName>
      <familyName>Valentini</familyName>
      <affiliation>International Center for Advanced Mediterranean Agronomic Studies (CIHEAM-IAMB), Bari, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>Fraser, Karen</creatorName>
      <givenName>Karen</givenName>
      <familyName>Fraser</familyName>
      <affiliation>Science and Advice for Scottish Agriculture (SASA), Edinburgh, United Kingdom</affiliation>
    </creator>
    <creator>
      <creatorName>Cara, Magdalena</creatorName>
      <givenName>Magdalena</givenName>
      <familyName>Cara</familyName>
      <affiliation>Agricultural University of Tirana (UBT), Tirana, Albania</affiliation>
    </creator>
    <creator>
      <creatorName>Yildiz, Nilufer</creatorName>
      <givenName>Nilufer</givenName>
      <familyName>Yildiz</familyName>
      <affiliation>Biological Control Research Institute (BCRI), Adana, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yuzbasioglu, Eda</creatorName>
      <givenName>Eda</givenName>
      <familyName>Yuzbasioglu</familyName>
      <affiliation>Biological Control Research Institute (BCRI), Adana, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Karahan, Aynur</creatorName>
      <givenName>Aynur</givenName>
      <familyName>Karahan</familyName>
      <affiliation>Plant Protection Central Research Institute, Ankara (PPCRI), Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ustun, Nursen</creatorName>
      <givenName>Nursen</givenName>
      <familyName>Ustun</familyName>
      <affiliation>Plant Protection Research Institute, Bornova (PPRI), Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kreuze, Jan</creatorName>
      <givenName>Jan</givenName>
      <familyName>Kreuze</familyName>
      <affiliation>International Potato Center (CIP), Lima, Peru</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <subjects>
    <subject>Euphresco, plant health, diagnostics, Ralstonia solanacearum, Ralstonia pseudosolanacearum, virulence genes, IFAS, real-time PCR, real-time LAMP</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2020-10-29</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Report"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4153357</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.4153356</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;The EU directive 2006/63/EC describes a detailed protocol for the official testing of &lt;em&gt;Ralstonia solanacearum&lt;/em&gt;, that is internationally recognized and has been implemented in many diagnostic laboratories across Europe and beyond. In this protocol, the confirmation of the identity of the bacterium is performed by a laborious, time-consuming and expensive pathogenicity test. Additionally, the described protocols involved in the official testing of &lt;em&gt;Ralstonia solanacearum&lt;/em&gt; (for detection and/or identification) require a lot of time, a high level of quarantine measures, and a high degree of expertise. The aim of this project was to develop, and evaluate molecular diagnostic methods for the detection and identification of &lt;em&gt;Ralstonia solanacearum&lt;/em&gt; and verification of its virulence that would be faster, more specific and robust. Based on the current taxonomic insights, the high degree of heterogenity present inside the &lt;em&gt;Ralstonia solanacearum&lt;/em&gt; species complex (RSSC) has resulted in the clear distinction of three new species inside this complex; the requirement defined in this Euphresco project was that the new molecular tests could be optimally implemented for the detection and identification of &lt;em&gt;Ralstonia solanacearum&lt;/em&gt; and &lt;em&gt;Ralstonia pseudosolanacearu&lt;/em&gt;m, but not of &lt;em&gt;Ralstonia syzygii&lt;/em&gt;.&lt;/p&gt;

&lt;p&gt;The two main objectives of the project were:&lt;/p&gt;

&lt;p&gt;1) To identify &lt;em&gt;Ralstonia solanacearum &lt;/em&gt;virulence genes, and subsequently develop a PCR test on those virulence genes. The development of such a test could substitute the pathogenicity test required by EU directive 2006/63/EC for complete diagnosis of &lt;em&gt;Ralstonia solanacearum&lt;/em&gt;.&lt;/p&gt;

&lt;p&gt;2) To verify other molecular methods to detect or identify &lt;em&gt;Ralstonia solanacearum &lt;/em&gt;strains. These methods include the real-time Loop-mediated isothermal amplication test (LAMP) and the recombinase-polymerase amplification assay (RPA).&lt;/p&gt;</description>
    <description descriptionType="Other">Report of the Euphresco project 2016-A-180 'Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence'</description>
  </descriptions>
</resource>
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