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Tjou-Tam-Sin, Napoleon; Vogelaar, Martijn; Li, XIang; Čermák, Vaclav; Fornefeld, Eva; van der Wolf, Jean Martin; Valentini, Franco; Fraser, Karen; Cara, Magdalena; Yildiz, Nilufer; Yuzbasioglu, Eda; Karahan, Aynur; Ustun, Nursen; Kreuze, Jan
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<dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://doi.org/10.5281/zenodo.4153357</dct:identifier> <foaf:page rdf:resource="https://doi.org/10.5281/zenodo.4153357"/> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Tjou-Tam-Sin, Napoleon</foaf:name> <foaf:givenName>Napoleon</foaf:givenName> <foaf:familyName>Tjou-Tam-Sin</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>National Reference Center, National Plant Protection Organisaton (NVWA), Wageningen, The Netherlands</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Vogelaar, Martijn</foaf:name> <foaf:givenName>Martijn</foaf:givenName> <foaf:familyName>Vogelaar</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>National Reference Center, National Plant Protection Organisaton (NVWA), Wageningen, The Netherlands</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Li, XIang</foaf:name> <foaf:givenName>XIang</foaf:givenName> <foaf:familyName>Li</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Canadian Food Inspection Agency (CFIA), Charlottetown, Canada</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Čermák, Vaclav</foaf:name> <foaf:givenName>Vaclav</foaf:givenName> <foaf:familyName>Čermák</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Central Institute for Supervising and Testing in Agriculture (ÚKZÜZ), Olomouc, Czech Republic</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Fornefeld, Eva</foaf:name> <foaf:givenName>Eva</foaf:givenName> <foaf:familyName>Fornefeld</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Julius Kühn-Institute, Federal Research Centre for Cultivated Plants (JKI), Kleinmachnow, Germany</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>van der Wolf, Jean Martin</foaf:name> <foaf:givenName>Jean Martin</foaf:givenName> <foaf:familyName>van der Wolf</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Wageningen Plant Research (WUR), Wageningen, the Netherlands</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Valentini, Franco</foaf:name> <foaf:givenName>Franco</foaf:givenName> <foaf:familyName>Valentini</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>International Center for Advanced Mediterranean Agronomic Studies (CIHEAM-IAMB), Bari, Italy</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Fraser, Karen</foaf:name> <foaf:givenName>Karen</foaf:givenName> <foaf:familyName>Fraser</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Science and Advice for Scottish Agriculture (SASA), Edinburgh, United Kingdom</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Cara, Magdalena</foaf:name> <foaf:givenName>Magdalena</foaf:givenName> <foaf:familyName>Cara</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Agricultural University of Tirana (UBT), Tirana, Albania</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Yildiz, Nilufer</foaf:name> <foaf:givenName>Nilufer</foaf:givenName> <foaf:familyName>Yildiz</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Biological Control Research Institute (BCRI), Adana, Turkey</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Yuzbasioglu, Eda</foaf:name> <foaf:givenName>Eda</foaf:givenName> <foaf:familyName>Yuzbasioglu</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Biological Control Research Institute (BCRI), Adana, Turkey</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Karahan, Aynur</foaf:name> <foaf:givenName>Aynur</foaf:givenName> <foaf:familyName>Karahan</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Plant Protection Central Research Institute, Ankara (PPCRI), Ankara, Turkey</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Ustun, Nursen</foaf:name> <foaf:givenName>Nursen</foaf:givenName> <foaf:familyName>Ustun</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>Plant Protection Research Institute, Bornova (PPRI), Izmir, Turkey</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <foaf:name>Kreuze, Jan</foaf:name> <foaf:givenName>Jan</foaf:givenName> <foaf:familyName>Kreuze</foaf:familyName> <org:memberOf> <foaf:Organization> <foaf:name>International Potato Center (CIP), Lima, Peru</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:title>Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence</dct:title> <dct:publisher> <foaf:Agent> <foaf:name>Zenodo</foaf:name> </foaf:Agent> </dct:publisher> <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2020</dct:issued> <dcat:keyword>Euphresco, plant health, diagnostics, Ralstonia solanacearum, Ralstonia pseudosolanacearum, virulence genes, IFAS, real-time PCR, real-time LAMP</dcat:keyword> <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2020-10-29</dct:issued> <dct:language rdf:resource="http://publications.europa.eu/resource/authority/language/ENG"/> <owl:sameAs rdf:resource="https://zenodo.org/record/4153357"/> <adms:identifier> <adms:Identifier> <skos:notation rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://zenodo.org/record/4153357</skos:notation> <adms:schemeAgency>url</adms:schemeAgency> </adms:Identifier> </adms:identifier> <dct:isVersionOf rdf:resource="https://doi.org/10.5281/zenodo.4153356"/> <dct:description><p>The EU directive 2006/63/EC describes a detailed protocol for the official testing of <em>Ralstonia solanacearum</em>, that is internationally recognized and has been implemented in many diagnostic laboratories across Europe and beyond. In this protocol, the confirmation of the identity of the bacterium is performed by a laborious, time-consuming and expensive pathogenicity test. Additionally, the described protocols involved in the official testing of <em>Ralstonia solanacearum</em> (for detection and/or identification) require a lot of time, a high level of quarantine measures, and a high degree of expertise. The aim of this project was to develop, and evaluate molecular diagnostic methods for the detection and identification of <em>Ralstonia solanacearum</em> and verification of its virulence that would be faster, more specific and robust. Based on the current taxonomic insights, the high degree of heterogenity present inside the <em>Ralstonia solanacearum</em> species complex (RSSC) has resulted in the clear distinction of three new species inside this complex; the requirement defined in this Euphresco project was that the new molecular tests could be optimally implemented for the detection and identification of <em>Ralstonia solanacearum</em> and <em>Ralstonia pseudosolanacearu</em>m, but not of <em>Ralstonia syzygii</em>.</p> <p>The two main objectives of the project were:</p> <p>1) To identify <em>Ralstonia solanacearum </em>virulence genes, and subsequently develop a PCR test on those virulence genes. The development of such a test could substitute the pathogenicity test required by EU directive 2006/63/EC for complete diagnosis of <em>Ralstonia solanacearum</em>.</p> <p>2) To verify other molecular methods to detect or identify <em>Ralstonia solanacearum </em>strains. These methods include the real-time Loop-mediated isothermal amplication test (LAMP) and the recombinase-polymerase amplification assay (RPA).</p></dct:description> <dct:description>Report of the Euphresco project 2016-A-180 'Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence'</dct:description> <dct:accessRights rdf:resource="http://publications.europa.eu/resource/authority/access-right/PUBLIC"/> <dct:accessRights> <dct:RightsStatement rdf:about="info:eu-repo/semantics/openAccess"> <rdfs:label>Open Access</rdfs:label> </dct:RightsStatement> </dct:accessRights> <dct:license rdf:resource="https://creativecommons.org/licenses/by/4.0/legalcode"/> <dcat:distribution> <dcat:Distribution> <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.4153357"/> <dcat:byteSize>570023</dcat:byteSize> <dcat:downloadURL rdf:resource="https://zenodo.org/record/4153357/files/report_2016-a-180_FINAL.pdf"/> <dcat:mediaType>application/pdf</dcat:mediaType> </dcat:Distribution> </dcat:distribution> </rdf:Description> </rdf:RDF>
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