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Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence

Tjou-Tam-Sin, Napoleon; Vogelaar, Martijn; Li, XIang; Čermák, Vaclav; Fornefeld, Eva; van der Wolf, Jean Martin; Valentini, Franco; Fraser, Karen; Cara, Magdalena; Yildiz, Nilufer; Yuzbasioglu, Eda; Karahan, Aynur; Ustun, Nursen; Kreuze, Jan


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.4153357", 
  "language": "eng", 
  "title": "Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence", 
  "issued": {
    "date-parts": [
      [
        2020, 
        10, 
        29
      ]
    ]
  }, 
  "abstract": "<p>The EU directive 2006/63/EC describes a detailed protocol for the official testing of <em>Ralstonia solanacearum</em>, that is internationally recognized and has been implemented in many diagnostic laboratories across Europe and beyond. In this protocol, the confirmation of the identity of the bacterium is performed by a laborious, time-consuming and expensive pathogenicity test. Additionally, the described protocols involved in the official testing of <em>Ralstonia solanacearum</em> (for detection and/or identification) require a lot of time, a high level of quarantine measures, and a high degree of expertise. The aim of this project was to develop, and evaluate molecular diagnostic methods for the detection and identification of <em>Ralstonia solanacearum</em> and verification of its virulence that would be faster, more specific and robust. Based on the current taxonomic insights, the high degree of heterogenity present inside the <em>Ralstonia solanacearum</em> species complex (RSSC) has resulted in the clear distinction of three new species inside this complex; the requirement defined in this Euphresco project was that the new molecular tests could be optimally implemented for the detection and identification of <em>Ralstonia solanacearum</em> and <em>Ralstonia pseudosolanacearu</em>m, but not of <em>Ralstonia syzygii</em>.</p>\n\n<p>The two main objectives of the project were:</p>\n\n<p>1) To identify <em>Ralstonia solanacearum </em>virulence genes, and subsequently develop a PCR test on those virulence genes. The development of such a test could substitute the pathogenicity test required by EU directive 2006/63/EC for complete diagnosis of <em>Ralstonia solanacearum</em>.</p>\n\n<p>2) To verify other molecular methods to detect or identify <em>Ralstonia solanacearum </em>strains. These methods include the real-time Loop-mediated isothermal amplication test (LAMP) and the recombinase-polymerase amplification assay (RPA).</p>", 
  "author": [
    {
      "family": "Tjou-Tam-Sin, Napoleon"
    }, 
    {
      "family": "Vogelaar, Martijn"
    }, 
    {
      "family": "Li, XIang"
    }, 
    {
      "family": "\u010cerm\u00e1k, Vaclav"
    }, 
    {
      "family": "Fornefeld, Eva"
    }, 
    {
      "family": "van der Wolf, Jean Martin"
    }, 
    {
      "family": "Valentini, Franco"
    }, 
    {
      "family": "Fraser, Karen"
    }, 
    {
      "family": "Cara, Magdalena"
    }, 
    {
      "family": "Yildiz, Nilufer"
    }, 
    {
      "family": "Yuzbasioglu, Eda"
    }, 
    {
      "family": "Karahan, Aynur"
    }, 
    {
      "family": "Ustun, Nursen"
    }, 
    {
      "family": "Kreuze, Jan"
    }
  ], 
  "note": "Report of the Euphresco project 2016-A-180 'Interlaboratory test performance studies for identification of Ralstonia solanacearum and molecular confirmation of its virulence'", 
  "type": "article", 
  "id": "4153357"
}
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