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HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species

Katja Kramberger; Darja Barlič-Maganja; Dunja Bandelj; Alenka Baruca Arbeiter; Kelly Peeters; Ana Miklavčič Višnjevec; Zala Jenko Pražnikar


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  <identifier identifierType="URL">https://zenodo.org/record/4104136</identifier>
  <creators>
    <creator>
      <creatorName>Katja Kramberger</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-4589-0644</nameIdentifier>
      <affiliation>Faculty of Health Sciences, University of Primorska, Slovenia; Faculty of Medicine, University of Ljubljana, Slovenia</affiliation>
    </creator>
    <creator>
      <creatorName>Darja Barlič-Maganja</creatorName>
      <affiliation>Faculty of Health Sciences, University of Primorska, Slovenia</affiliation>
    </creator>
    <creator>
      <creatorName>Dunja Bandelj</creatorName>
      <affiliation>Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Slovenia</affiliation>
    </creator>
    <creator>
      <creatorName>Alenka Baruca Arbeiter</creatorName>
      <affiliation>Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Slovenia</affiliation>
    </creator>
    <creator>
      <creatorName>Kelly Peeters</creatorName>
      <affiliation>InnoRenew CoE, Slovenia; Andrej Marušič Institute, University of Primorska, Slovenia</affiliation>
    </creator>
    <creator>
      <creatorName>Ana Miklavčič Višnjevec</creatorName>
      <affiliation>Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Slovenia</affiliation>
    </creator>
    <creator>
      <creatorName>Zala Jenko Pražnikar</creatorName>
      <affiliation>Faculty of Health Sciences, University of Primorska, Slovenia</affiliation>
    </creator>
  </creators>
  <titles>
    <title>HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <subjects>
    <subject>H. italicum</subject>
    <subject>plant extracts</subject>
    <subject>phenolic compounds</subject>
    <subject>antioxidant activity</subject>
    <subject>HPLC-DAD-ESI-QTOF-MS</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2020-10-12</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="issn">2218-1989</alternateIdentifier>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4104136</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3390/metabo10100403</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/innorenew</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Mediterranean plant&amp;nbsp;Helichrysum italicum&amp;nbsp;represents a rich source of versatile bioactive compounds with potential benefits for human health. Despite extensive research on the plant&amp;rsquo;s active constituents, little attention has yet been paid to characterizing the relationship between its intra-specific genetic diversity and metabolite profile. The study aimed to determine metabolic profile of&amp;nbsp;H. italicum&amp;nbsp;ssp.&amp;nbsp;italicum&amp;nbsp;(HII) and ssp.&amp;nbsp;tyrrhenicum&amp;nbsp;(HIT) cultivated on the experimental plantation in Slovenia and to compare the chemical composition of extracts regarding the solvent extraction process. Extracts were prepared upon conventional extract preparation procedures: maceration with 50% methanol or ethanol and cold or hot water infusion and analyzed using High Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization-Quadrupole Time-of-Flight-Mass Spectrometry (HPLC-DAD-ESI-QTOF-MS). One hundred compounds were identified in the samples, among them several isomers and derivatives were reported for the first time, while caffeoylquinic acids and pyrones were the most abundant. Semi-quantitative comparison revealed that the extraction procedure had a greater impact on the chemical profile than genetic variability. All&amp;nbsp;HIT&amp;nbsp;extracts showed a higher total phenolic content compared to&amp;nbsp;HII, while the antioxidant potential evaluated by 1,1-diphenyl-2-picrylhydrazil test was not proportionally higher. In addition, hot water extracts proved to be comparably active as alcoholic ones, confirming high commercial potential of&amp;nbsp;Helichrysum italicum&amp;nbsp;as herbal functional beverages.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/739574/">739574</awardNumber>
      <awardTitle>Renewable materials and healthy environments research and innovation centre of excellence</awardTitle>
    </fundingReference>
  </fundingReferences>
</resource>
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