Journal article Open Access

HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species

Katja Kramberger; Darja Barlič-Maganja; Dunja Bandelj; Alenka Baruca Arbeiter; Kelly Peeters; Ana Miklavčič Višnjevec; Zala Jenko Pražnikar


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{
  "DOI": "10.3390/metabo10100403", 
  "container_title": "Metabolites", 
  "title": "HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species", 
  "issued": {
    "date-parts": [
      [
        2020, 
        10, 
        12
      ]
    ]
  }, 
  "abstract": "<p>Mediterranean plant&nbsp;Helichrysum italicum&nbsp;represents a rich source of versatile bioactive compounds with potential benefits for human health. Despite extensive research on the plant&rsquo;s active constituents, little attention has yet been paid to characterizing the relationship between its intra-specific genetic diversity and metabolite profile. The study aimed to determine metabolic profile of&nbsp;H. italicum&nbsp;ssp.&nbsp;italicum&nbsp;(HII) and ssp.&nbsp;tyrrhenicum&nbsp;(HIT) cultivated on the experimental plantation in Slovenia and to compare the chemical composition of extracts regarding the solvent extraction process. Extracts were prepared upon conventional extract preparation procedures: maceration with 50% methanol or ethanol and cold or hot water infusion and analyzed using High Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization-Quadrupole Time-of-Flight-Mass Spectrometry (HPLC-DAD-ESI-QTOF-MS). One hundred compounds were identified in the samples, among them several isomers and derivatives were reported for the first time, while caffeoylquinic acids and pyrones were the most abundant. Semi-quantitative comparison revealed that the extraction procedure had a greater impact on the chemical profile than genetic variability. All&nbsp;HIT&nbsp;extracts showed a higher total phenolic content compared to&nbsp;HII, while the antioxidant potential evaluated by 1,1-diphenyl-2-picrylhydrazil test was not proportionally higher. In addition, hot water extracts proved to be comparably active as alcoholic ones, confirming high commercial potential of&nbsp;Helichrysum italicum&nbsp;as herbal functional beverages.</p>", 
  "ISSN": "2218-1989", 
  "volume": "10", 
  "author": [
    {
      "family": "Katja Kramberger"
    }, 
    {
      "family": "Darja Barli\u010d-Maganja"
    }, 
    {
      "family": "Dunja Bandelj"
    }, 
    {
      "family": "Alenka Baruca Arbeiter"
    }, 
    {
      "family": "Kelly Peeters"
    }, 
    {
      "family": "Ana Miklav\u010di\u010d Vi\u0161njevec"
    }, 
    {
      "family": "Zala Jenko Pra\u017enikar"
    }
  ], 
  "type": "article-journal", 
  "issue": "10", 
  "id": "4104136"
}
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