Preprint Open Access
Rafael Piñol; Justyna Zeler; Carlos D. S. Brites; Yuanyu Gu; Pedro Téllez; Albano N. Carneiro Neto; Thiago E. da Silva; Raquel Moreno-Loshuertos; Patricio Fernandez-Silva; Ana Gallego; Luis Martinez-Lostao; Abelardo Martínez; Luís D. Carlos; Angel Millán
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln=Sm, Eu) incubated in breast cancer MDA-MB468 cells. 2D thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ~20 degrees and subcellular gradients of ~5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.