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Anomalies in BatCoV/RaTG13 sequencing and provenance

Daoyu Zhang


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  <identifier identifierType="DOI">10.5281/zenodo.4008432</identifier>
  <creators>
    <creator>
      <creatorName>Daoyu Zhang</creatorName>
      <affiliation>The University Of Sydney</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Anomalies in BatCoV/RaTG13 sequencing and provenance</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2020</publicationYear>
  <subjects>
    <subject>RaTG13</subject>
    <subject>Raw Data</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2020-08-01</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Report</resourceType>
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    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/4008432</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3969271</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/covid-19</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;To this date, the most critical piece of evidence on the purposed &amp;ldquo;natural origin&amp;rdquo; theory of SARS-CoV-2, was the sequence known as RaTG13, allegedly collected from a single fecal sample from Rhinolophus Affinis. Understanding the provenance of RaTG13 is critical on the ongoing debate of the Origins of SARS-CoV-2. However, this sample is allegedly &amp;ldquo;used up&amp;rdquo; and therefore can no longer be accessed nor sequenced independently [1], and the only available data was the 3 related Genbank accessions: MN996532.1, SRX7724752 and SRX8357956.&lt;/p&gt;

&lt;p&gt;We report these datasets possessed multiple significant anomalies, and the provenence of the promised claims of RaTG13 or it&amp;rsquo;s role in proving a &amp;ldquo;probable bat origin&amp;rdquo;[2] of SARS-CoV-2 can not be satisfied nor possibly be confirmed.&lt;/p&gt;</description>
    <description descriptionType="Other">{"references": ["Zhou P, Yang XL, Wang XG, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579(7798):270-273. doi:10.1038/s41586-020-2012-7", "Rose C, Parker A, Jefferson B, Cartmell E. The Characterization of Feces and Urine: A Review of the Literature to Inform Advanced Treatment Technology. Crit Rev Environ Sci Technol. 2015;45(17):1827-1879. doi:10.1080/10643389.2014.1000761", "Xiao C, Li X, Liu S, Sang Y, Gao SJ, Gao F. HIV-1 did not contribute to the 2019-nCoV genome. Emerg Microbes Infect. 2020;9(1):378-381. Published 2020 Feb 14. doi:10.1080/22221751.2020.1727299", "Paskey, A.C., Frey, K.G., Schroth, G. et al. Enrichment post-library preparation enhances the sensitivity of high-throughput sequencing-based detection and characterization of viruses from complex samples. BMC Genomics 20, 155 (2019). https://doi.org/10.1186/s12864-019-5543-2"]}</description>
  </descriptions>
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