Journal article Open Access

Proteome consistency of the aleurone layer in grain of major wheat species grown over different years

Meziani, Samira; Nadaud, Isabelle


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p><strong>Background: </strong>Aleurone layer (AL), being a living cell layer among the peripheral layers of the grain structure obtained after milling wheat, is rich in, vitamins, minerals, and antioxidants potentially nutritional value of the flour. <strong>Objectives: </strong>To isolate AL in the mature grain of the three major species; Common wheat (CW)<em>, </em>Durum wheat (DW), and Einkorn wheat (EW) that were grown at two different years as well as to analyze and compare their proteomes revealed through two-dimensional electrophoresis (2DE) and image analysis. <strong>Methods</strong>: The AL was hand dissected and unicellular purity verified using scanning electron microscopy. AL proteins were separated using IEF pH3-10 X SDS-PAGE then Coomassie-stained. The gels were scanned and the images were compared using Samespot (Nonlinear Dynamics) and were proteins identified using mass spectrometry and database interrogation. <strong>Results</strong>: For CW and DW samples, no significant quantitative or qualitative differences were observed between the AL proteome in the two years. However, a few quantitative differences were revealed between EW for AL of 2006 and 2007. The &nbsp;identified proteins were classified in the carbohydrate pathway and stress defense response. <strong>Conclusion:</strong> This remarkable stability over environmental growing conditions strengthens the need to pay greater attention to this unicellular living cell of the wheat grain.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Laboratory of Biotoxicology, Djillali Liabes University, Faculty of Life and Natural Sciences, Department of Biology, Algeria", 
      "@id": "https://orcid.org/0000-0002-4027-9670", 
      "@type": "Person", 
      "name": "Meziani, Samira"
    }, 
    {
      "affiliation": "UMR. INRA GDEC-UCA 1095, 5 Chemin de Beaulieu, 63000 Clermont-Ferrand, France", 
      "@type": "Person", 
      "name": "Nadaud, Isabelle"
    }
  ], 
  "headline": "Proteome consistency of the aleurone layer in grain of major wheat species grown over different years", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2020-08-11", 
  "url": "https://zenodo.org/record/3974052", 
  "keywords": [
    "Wheat", 
    "durum", 
    "einkorn", 
    "aleurone layer", 
    "metabolic pathways"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.3974052", 
  "@id": "https://doi.org/10.5281/zenodo.3974052", 
  "@type": "ScholarlyArticle", 
  "name": "Proteome consistency of the aleurone layer in grain of major wheat species grown over different years"
}
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